Glucose Metabolism in Lactococcus lactis MG1363 under Different Aeration Conditions: Requirement of Acetate To Sustain Growth under Microaerobic Conditions Export

@article{citeulike:2923968, abstract = {Lactococcus lactis subsp. lactis MG1363 was grown in batch cultures on a defined medium with glucose as the energy source under different aeration conditions, namely, anaerobic conditions, aerobic conditions, and microaerobic conditions with a dissolved oxygen tension of 5\% (when saturation with air was used as the reference). The maximum specific growth rate was high (0.78 to 0.91 h-1) under all aeration conditions but decreased with increasing aeration, and more than 90\% of the glucose was converted to lactate. However, a shift in by-product formation was observed. Increasing aeration resulted in acetate, CO2, and acetoin replacing formate and ethanol as end products. Under microaerobic conditions, growth came to a gradual halt, although more than 60\% of the glucose was still left. A decline in growth was not observed during microaerobic cultivation when acetate was added to the medium. We hypothesize that the decline in growth was due to a lack of acetyl coenzyme A (acetyl-CoA) needed for fatty acid synthesis since acetyl-CoA can be synthesized from acetate by means of acetate kinase and phosphotransacetylase activities. 10.1128/AEM.69.6.3462-3468.2003}, author = {Nordkvist, Mikkel and Jensen, Niels B. and Villadsen, John}, citeulike-article-id = {2923968}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/AEM.69.6.3462-3468.2003}, citeulike-linkout-1 = {http://aem.asm.org/cgi/content/abstract/69/6/3462}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12788751}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12788751}, day = {1}, doi = {10.1128/AEM.69.6.3462-3468.2003}, journal = {Appl. Environ. Microbiol.}, keywords = {acetate, glucose, lactis, lactococcus, metabolism, mg1363, microaerobic, printed}, month = {June}, number = {6}, pages = {3462--3468}, posted-at = {2008-06-24 17:27:20}, priority = {2}, title = {Glucose Metabolism in Lactococcus lactis MG1363 under Different Aeration Conditions: Requirement of Acetate To Sustain Growth under Microaerobic Conditions}, url = {http://dx.doi.org/10.1128/AEM.69.6.3462-3468.2003}, volume = {69}, year = {2003} }

TY - JOUR ID - citeulike:2923968 L3 - citeulike-article-id:2923968 N2 - Lactococcus lactis subsp. lactis MG1363 was grown in batch cultures on a defined medium with glucose as the energy source under different aeration conditions, namely, anaerobic conditions, aerobic conditions, and microaerobic conditions with a dissolved oxygen tension of 5% (when saturation with air was used as the reference). The maximum specific growth rate was high (0.78 to 0.91 h-1) under all aeration conditions but decreased with increasing aeration, and more than 90% of the glucose was converted to lactate. However, a shift in by-product formation was observed. Increasing aeration resulted in acetate, CO2, and acetoin replacing formate and ethanol as end products. Under microaerobic conditions, growth came to a gradual halt, although more than 60% of the glucose was still left. A decline in growth was not observed during microaerobic cultivation when acetate was added to the medium. We hypothesize that the decline in growth was due to a lack of acetyl coenzyme A (acetyl-CoA) needed for fatty acid synthesis since acetyl-CoA can be synthesized from acetate by means of acetate kinase and phosphotransacetylase activities. 10.1128/AEM.69.6.3462-3468.2003 IS - 6 JF - Appl. Environ. Microbiol. EP - 3468 TI - Glucose Metabolism in Lactococcus lactis MG1363 under Different Aeration Conditions: Requirement of Acetate To Sustain Growth under Microaerobic Conditions VL - 69 SP - 3462 KW - acetate KW - glucose KW - lactis KW - lactococcus KW - metabolism KW - mg1363 KW - microaerobic KW - printed AU - Nordkvist, Mikkel AU - Jensen, Niels AU - Villadsen, John PY - 2003/06/01/ UR - http://dx.doi.org/10.1128/AEM.69.6.3462-3468.2003 ER -