The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains Export

@article{citeulike:3385648, abstract = {Disruption of the ZWF1 gene encoding glucose-6-phosphate dehydrogenase (G6PDH) has been shown to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain TMB3255. In the present investigation we have studied the influence of different production levels of G6PDH on xylose fermentation. We used a synthetic promoter library and the copper-regulated CUP1 promoter to generate G6PDH-activities between 0\% and 179\% of the wild-type level. G6PDH-activities of 1\% and 6\% of the wild-type level resulted in 2.8- and 5.1-fold increase in specific xylose consumption, respectively, compared with the ZWF1-disrupted strain. Both strains exhibited decreased xylitol yields (0.13 and 0.19 g/g xylose) and enhanced ethanol yields (0.36 and 0.34 g/g xylose) compared with the control strain TMB3001 (0.29 g xylitol/g xylose, 0.31 g ethanol/g xylose). Cytoplasmic transhydrogenase (TH) from Azotobacter vinelandii has previously been shown to transfer NADPH and NAD+ into NADP+ and NADH, and TH-overproduction resulted in lower xylitol yield and enhanced glycerol yield during xylose utilization. Strains with low G6PDH-activity grew slower in a lignocellulose hydrolysate than the strain with wild-type G6PDH-activity, which suggested that the availability of intracellular NADPH correlated with tolerance towards lignocellulose-derived inhibitors. Low G6PDH-activity strains were also more sensitive to H2O2 than the control strain TMB3001. Copyright {\copyright} 2003 John Wiley \& Sons, Ltd.}, author = {Jeppsson, Marie and Johansson, Bj\"{o}rn and Jensen, Peter R. and Hahn-H\"{a}gerdal, B\"{a}rbel and Gorwa-Grauslund, Marie F.}, citeulike-article-id = {3385648}, citeulike-linkout-0 = {http://www3.interscience.wiley.com/journal/106559100/abstract?CRETRY=1&SRETRY=0}, journal = {Yeast}, keywords = {cerevisiae, fermentation, glucose6phosphate\_dehydrogenase, inhibitors, nadph, redox\_balance, saccharomyces, synthetic\_promoter\_library, xylitol, xylose}, month = {November}, number = {15}, pages = {1263--1272}, posted-at = {2008-10-08 09:39:30}, priority = {2}, title = {The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains}, url = {http://www3.interscience.wiley.com/journal/106559100/abstract?CRETRY=1&SRETRY=0}, volume = {20}, year = {2003} }

TY - JOUR ID - citeulike:3385648 L3 - citeulike-article-id:3385648 N2 - Disruption of the ZWF1 gene encoding glucose-6-phosphate dehydrogenase (G6PDH) has been shown to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain TMB3255. In the present investigation we have studied the influence of different production levels of G6PDH on xylose fermentation. We used a synthetic promoter library and the copper-regulated CUP1 promoter to generate G6PDH-activities between 0% and 179% of the wild-type level. G6PDH-activities of 1% and 6% of the wild-type level resulted in 2.8- and 5.1-fold increase in specific xylose consumption, respectively, compared with the ZWF1-disrupted strain. Both strains exhibited decreased xylitol yields (0.13 and 0.19 g/g xylose) and enhanced ethanol yields (0.36 and 0.34 g/g xylose) compared with the control strain TMB3001 (0.29 g xylitol/g xylose, 0.31 g ethanol/g xylose). Cytoplasmic transhydrogenase (TH) from Azotobacter vinelandii has previously been shown to transfer NADPH and NAD+ into NADP+ and NADH, and TH-overproduction resulted in lower xylitol yield and enhanced glycerol yield during xylose utilization. Strains with low G6PDH-activity grew slower in a lignocellulose hydrolysate than the strain with wild-type G6PDH-activity, which suggested that the availability of intracellular NADPH correlated with tolerance towards lignocellulose-derived inhibitors. Low G6PDH-activity strains were also more sensitive to H2O2 than the control strain TMB3001. Copyright © 2003 John Wiley & Sons, Ltd. IS - 15 JF - Yeast EP - 1272 TI - The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains VL - 20 SP - 1263 KW - cerevisiae KW - fermentation KW - glucose6phosphate_dehydrogenase KW - inhibitors KW - nadph KW - redox_balance KW - saccharomyces KW - synthetic_promoter_library KW - xylitol KW - xylose AU - Jeppsson, Marie AU - Johansson, Björn AU - Jensen, Peter AU - Hahn-Hägerdal, Bärbel AU - Gorwa-Grauslund, Marie PY - 2003/11// UR - http://www3.interscience.wiley.com/journal/106559100/abstract?CRETRY=1&SRETRY=0 ER -