The Extent to Which ATP Demand Controls the Glycolytic Flux Depends Strongly on the Organism and Conditions for Growth

@article{citeulike:3385661, abstract = {Using molecular genetics we have introduced uncoupled ATPase activity in two different bacterial species, Escherichia coli and Lactococcus lactis, and determined the elasticities of the growth rate and glycolytic flux towards the intracellular [ATP]/[ADP] ratio. During balanced growth in batch cultures of E. coli the ATP demand was found to have almost full control on the glycolytic flux (FCC=0.96) and the flux could be stimulated by 70\%. In contrast to this, in L. lactis the control by ATP demand on the glycolytic flux was close to zero. However, when we used non-growing cells of L. lactis (which have a low glycolytic flux) the ATP demand had a high flux control and the flux could be stimulated more than two fold. We suggest that the extent to which ATP demand controls the glycolytic flux depends on how much excess capacity of glycolysis is present in the cells.}, author = {Koebmann, Brian J. and Westerhoff, Hans V. and Snoep, Jacky L. and Solem, Christian and Pedersen, Martin B. and Nilsson, Dan and Michelsen, Ole and Jensen, Peter R.}, citeulike-article-id = {3385661}, doi = {10.1023/A:1020398117281}, journal = {Molecular Biology Reports}, keywords = {atp, conditions, control, demand, flux, glycolytic, growth, organism}, month = {March}, number = {1}, pages = {41--45}, posted-at = {2008-10-08 09:46:32}, priority = {3}, title = {The Extent to Which ATP Demand Controls the Glycolytic Flux Depends Strongly on the Organism and Conditions for Growth}, url = {http://dx.doi.org/10.1023/A:1020398117281}, volume = {29}, year = {2002} }

TY - JOUR ID - citeulike:3385661 L3 - citeulike-article-id:3385661 N2 - Using molecular genetics we have introduced uncoupled ATPase activity in two different bacterial species, Escherichia coli and Lactococcus lactis, and determined the elasticities of the growth rate and glycolytic flux towards the intracellular [ATP]/[ADP] ratio. During balanced growth in batch cultures of E. coli the ATP demand was found to have almost full control on the glycolytic flux (FCC=0.96) and the flux could be stimulated by 70%. In contrast to this, in L. lactis the control by ATP demand on the glycolytic flux was close to zero. However, when we used non-growing cells of L. lactis (which have a low glycolytic flux) the ATP demand had a high flux control and the flux could be stimulated more than two fold. We suggest that the extent to which ATP demand controls the glycolytic flux depends on how much excess capacity of glycolysis is present in the cells. IS - 1 JF - Molecular Biology Reports EP - 45 TI - The Extent to Which ATP Demand Controls the Glycolytic Flux Depends Strongly on the Organism and Conditions for Growth VL - 29 SP - 41 KW - atp KW - conditions KW - control KW - demand KW - flux KW - glycolytic KW - growth KW - organism AU - Koebmann, Brian AU - Westerhoff, Hans AU - Snoep, Jacky AU - Solem, Christian AU - Pedersen, Martin AU - Nilsson, Dan AU - Michelsen, Ole AU - Jensen, Peter PY - 2002/03/01/ UR - http://dx.doi.org/10.1023/A:1020398117281 ER -