Essential genes of a minimal bacterium Export

@article{glass06, abstract = {Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be a close approximation to the minimal set of genes needed to sustain bacterial life. Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein-coding genes. None of the 43 RNA-coding genes were disrupted. Herein, we identify 382 of the 482 M. genitalium protein-coding genes as essential, plus five sets of disrupted genes that encode proteins with potentially redundant essential functions, such as phosphate transport. Genes encoding proteins of unknown function constitute 28\% of the essential protein-coding genes set. Disruption of some genes accelerated M. genitalium growth.}, author = {Glass, John I. and Assad, Nacyra and Garcia and Alperovich, Nina and Yooseph, Shibu and Lewis, Matthew R. and Maruf, Mahir and Hutchison and Ii, I. and Clyde, A. and Smith, Hamilton O. and Venter, Craig J.}, citeulike-article-id = {1570316}, doi = {10.1073/pnas.0510013103}, journal = {Proc. Natl. Acad. Sci. USA}, keywords = {minimal, mutagenesis, mycoplasma, syntheticbiology, transposon}, month = {January}, number = {2}, pages = {425--430}, posted-at = {2007-08-16 21:26:57}, priority = {0}, title = {Essential genes of a minimal bacterium}, volume = {103}, year = {2006} }

TY - JOUR ID - glass06 L3 - citeulike-article-id:1570316 N2 - Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be a close approximation to the minimal set of genes needed to sustain bacterial life. Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein-coding genes. None of the 43 RNA-coding genes were disrupted. Herein, we identify 382 of the 482 M. genitalium protein-coding genes as essential, plus five sets of disrupted genes that encode proteins with potentially redundant essential functions, such as phosphate transport. Genes encoding proteins of unknown function constitute 28\% of the essential protein-coding genes set. Disruption of some genes accelerated M. genitalium growth. IS - 2 JF - Proc. Natl. Acad. Sci. USA EP - 430 TI - Essential genes of a minimal bacterium VL - 103 SP - 425 KW - minimal KW - mutagenesis KW - mycoplasma KW - syntheticbiology KW - transposon AU - Glass, John AU - Assad, Nacyra AU - Garcia AU - Alperovich, Nina AU - Yooseph, Shibu AU - Lewis, Matthew AU - Maruf, Mahir AU - Hutchison AU - Ii, I AU - Clyde, A AU - Smith, Hamilton AU - Venter, Craig PY - 2006/January// ER -