Real-time RNA profiling within a single bacterium Export

@article{le05, abstract = {Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium of Escherichia coli exhibited a pulsating profile in response to a steady input of inducer. The genetic deletion of an efflux pump system, a key determinant of antibiotic resistance, altered the pulsating transcriptional dynamics and caused overexpression of induced RNA. In contrast with population measurements, real-time RNA profiling permits identifying relationships between genotypes and transcriptional dynamics that are accessible only at the level of the single cell.}, annote = {MS2 RNA binding}, author = {Le, Thuc T. and Harlepp, S\'ebastien and Guet, Calin C. and Kimberly and Pan, Tao and Cluzel, Philippe}, citeulike-article-id = {1570472}, doi = {doi:10.1073/pnas.0503311102}, journal = {Proc. Natl. Acad. Sci. USA}, keywords = {cell\_cycle, correlation, fluorescence, multidrug, noise, spectroscopy, transcription}, month = {June}, number = {26}, pages = {9160--9164}, posted-at = {2007-08-16 21:27:02}, priority = {0}, title = {Real-time {RNA} profiling within a single bacterium}, volume = {102}, year = {2005} }

TY - JOUR ID - le05 L3 - citeulike-article-id:1570472 N2 - Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium of Escherichia coli exhibited a pulsating profile in response to a steady input of inducer. The genetic deletion of an efflux pump system, a key determinant of antibiotic resistance, altered the pulsating transcriptional dynamics and caused overexpression of induced RNA. In contrast with population measurements, real-time RNA profiling permits identifying relationships between genotypes and transcriptional dynamics that are accessible only at the level of the single cell. IS - 26 JF - Proc. Natl. Acad. Sci. USA EP - 9164 TI - Real-time {RNA} profiling within a single bacterium VL - 102 SP - 9160 KW - cell_cycle KW - correlation KW - fluorescence KW - multidrug KW - noise KW - spectroscopy KW - transcription AU - Le, Thuc AU - Harlepp, S\'ebastien AU - Guet, Calin AU - Kimberly AU - Pan, Tao AU - Cluzel, Philippe PY - 2005/June// ER -