Generation of a Bacterium with a 21 Amino Acid Genetic Code Export

@article{mehl03, abstract = {We have generated a completely autonomous bacterium with a 21 amino acid genetic code. This bacterium can biosynthesize a nonstandard amino acid from basic carbon sources and incorporate this amino acid into proteins in response to the amber nonsense codon. The biosynthetic pathway for the amino acid p-aminophenylalanine (pAF) as well as a unique pAF synthetase and cognate tRNA were added to Escherichia coli. Denaturing gel electrophoresis and mass spectrometric analysis show that pAF is incorporated into myoglobin with fidelity and efficiency rivaling those of the common 20 amino acids. This and other such organisms may provide an opportunity to examine the evolutionary consequences of adding new amino acids to the genetic repertoire, as well as generate proteins with new or enhanced biological functions.}, author = {Mehl, Ryan A. and Anderson, Christopher J. and Santoro, Stephen W. and Wang, Lei and Martin, Andrew B. and King, David S. and Horn, David M. and Schultz, Peter G.}, citeulike-article-id = {1570547}, doi = {doi:10.1021/ja0284153}, journal = {JACS}, number = {4}, pages = {935--939}, posted-at = {2007-08-16 21:27:03}, priority = {0}, title = {Generation of a Bacterium with a 21 Amino Acid Genetic Code}, volume = {125}, year = {2003} }

TY - JOUR ID - mehl03 L3 - citeulike-article-id:1570547 N2 - We have generated a completely autonomous bacterium with a 21 amino acid genetic code. This bacterium can biosynthesize a nonstandard amino acid from basic carbon sources and incorporate this amino acid into proteins in response to the amber nonsense codon. The biosynthetic pathway for the amino acid p-aminophenylalanine (pAF) as well as a unique pAF synthetase and cognate tRNA were added to Escherichia coli. Denaturing gel electrophoresis and mass spectrometric analysis show that pAF is incorporated into myoglobin with fidelity and efficiency rivaling those of the common 20 amino acids. This and other such organisms may provide an opportunity to examine the evolutionary consequences of adding new amino acids to the genetic repertoire, as well as generate proteins with new or enhanced biological functions. IS - 4 JF - JACS EP - 939 TI - Generation of a Bacterium with a 21 Amino Acid Genetic Code VL - 125 SP - 935 AU - Mehl, Ryan AU - Anderson, Christopher AU - Santoro, Stephen AU - Wang, Lei AU - Martin, Andrew AU - King, David AU - Horn, David AU - Schultz, Peter PY - 2003/// ER -