Doubly Catalytic Sensing of HIV-1-Related CCR5 Sequence in Prokaryotic Cell-Free Translation System using Riboregulator-Controlled Luciferase Activity TeX Export

@article{sando05, abstract = {A molecular-beacon-type riboregulator (mRNA) was applied to multiply catalytic gene sensing. It consists of a reporter gene for firefly protein luciferase and, upstream thereof, a regulator hairpin domain composed of an RBS/anti-RBS stem (RBS = ribosome binding site) and a loop which is complementary to the target. The hairpin and, hence, the RBS are rendered open upon binding of a target oligonucleotide of the human CC chemokine receptor 5 sequence in a prokaryotic cell-free translation system (10 \$\mu\$L) to ignite ribosomal catalytic translation, or transcription/translation when using a DNA form of the probe, to produce luciferase, which is assayed by a catalytic chemiluminescence reaction. The sensing, using an unmodified RNA or even dsDNA as a probe with a chemiluminescence output, is thus doubly catalytic or amplifiable with a sensitivity at <=50 fmol in respect to the target with 4.5 fmol (1 ng/\$\mu\$L) of probe and a single nucleotide resolution.}, author = {Sando, Shinsuke and Narita, Atsushi and Abe, Kenji and Aoyama, Yasuhiro}, citeulike-article-id = {1570672}, doi = {doi:10.1021/ja0507057}, journal = {JACS}, number = {15}, pages = {5300--5301}, posted-at = {2007-08-16 21:27:05}, priority = {0}, title = {Doubly Catalytic Sensing of {HIV}-1-Related {CCR5} Sequence in Prokaryotic Cell-Free Translation System using Riboregulator-Controlled Luciferase Activity}, volume = {127}, year = {2005} }

TY - JOUR ID - sando05 L3 - citeulike-article-id:1570672 N2 - A molecular-beacon-type riboregulator (mRNA) was applied to multiply catalytic gene sensing. It consists of a reporter gene for firefly protein luciferase and, upstream thereof, a regulator hairpin domain composed of an RBS/anti-RBS stem (RBS = ribosome binding site) and a loop which is complementary to the target. The hairpin and, hence, the RBS are rendered open upon binding of a target oligonucleotide of the human CC chemokine receptor 5 sequence in a prokaryotic cell-free translation system (10 $\mu$L) to ignite ribosomal catalytic translation, or transcription/translation when using a DNA form of the probe, to produce luciferase, which is assayed by a catalytic chemiluminescence reaction. The sensing, using an unmodified RNA or even dsDNA as a probe with a chemiluminescence output, is thus doubly catalytic or amplifiable with a sensitivity at <=50 fmol in respect to the target with 4.5 fmol (1 ng/$\mu$L) of probe and a single nucleotide resolution. IS - 15 JF - JACS EP - 5301 TI - Doubly Catalytic Sensing of {HIV}-1-Related {CCR5} Sequence in Prokaryotic Cell-Free Translation System using Riboregulator-Controlled Luciferase Activity VL - 127 SP - 5300 AU - Sando, Shinsuke AU - Narita, Atsushi AU - Abe, Kenji AU - Aoyama, Yasuhiro PY - 2005/// ER -