Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis. Export

@article{citeulike:2540217, abstract = {The emerging field of synthetic genomics is expected to facilitate the generation of microorganisms with the potential to achieve a sustainable society. One approach towards this goal is the reduction of microbial genomes by rationally designed deletions to create simplified cells with predictable behavior that act as a platform to build in various genetic systems for specific purposes. We report a novel Bacillus subtilis strain, MBG874, depleted of 874 kb (20\%) of the genomic sequence. When compared with wild-type cells, the regulatory network of gene expression of the mutant strain is reorganized after entry into the transition state due to the synergistic effect of multiple deletions, and productivity of extracellular cellulase and protease from transformed plasmids harboring the corresponding genes is remarkably enhanced. To our knowledge, this is the first report demonstrating that genome reduction actually contributes to the creation of bacterial cells with a practical application in industry. Further systematic analysis of changes in the transcriptional regulatory network of MGB874 cells in relation to protein productivity should facilitate the generation of improved B. subtilis cells as hosts of industrial protein production.}, address = {1Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan.}, author = {Morimoto, Takuya and Kadoya, Ryosuke and Endo, Keiji and Tohata, Masatoshi and Sawada, Kazuhisa and Liu, Shengao and Ozawa, Tadahiro and Kodama, Takeko and Kakeshita, Hiroshi and Kageyama, Yasushi and Manabe, Kenji and Kanaya, Shigehiko and Ara, Katsutoshi and Ozaki, Katsuya and Ogasawara, Naotake}, citeulike-article-id = {2540217}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/dnares/dsn002}, citeulike-linkout-1 = {http://dnaresearch.oxfordjournals.org/cgi/content/abstract/15/2/73}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18334513}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18334513}, day = {11}, doi = {10.1093/dnares/dsn002}, issn = {1340-2838}, journal = {DNA Res}, keywords = {bacillus, expression, genome, industrial, metabolic, reduction, subtilis}, month = {March}, posted-at = {2008-06-11 02:47:55}, priority = {0}, title = {Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis.}, url = {http://dx.doi.org/10.1093/dnares/dsn002}, year = {2008} }

TY - JOUR ID - citeulike:2540217 L3 - citeulike-article-id:2540217 N2 - The emerging field of synthetic genomics is expected to facilitate the generation of microorganisms with the potential to achieve a sustainable society. One approach towards this goal is the reduction of microbial genomes by rationally designed deletions to create simplified cells with predictable behavior that act as a platform to build in various genetic systems for specific purposes. We report a novel Bacillus subtilis strain, MBG874, depleted of 874 kb (20%) of the genomic sequence. When compared with wild-type cells, the regulatory network of gene expression of the mutant strain is reorganized after entry into the transition state due to the synergistic effect of multiple deletions, and productivity of extracellular cellulase and protease from transformed plasmids harboring the corresponding genes is remarkably enhanced. To our knowledge, this is the first report demonstrating that genome reduction actually contributes to the creation of bacterial cells with a practical application in industry. Further systematic analysis of changes in the transcriptional regulatory network of MGB874 cells in relation to protein productivity should facilitate the generation of improved B. subtilis cells as hosts of industrial protein production. JF - DNA Res SN - 1340-2838 AD - 1Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan. TI - Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis. KW - bacillus KW - expression KW - genome KW - industrial KW - metabolic KW - reduction KW - subtilis AU - Morimoto, Takuya AU - Kadoya, Ryosuke AU - Endo, Keiji AU - Tohata, Masatoshi AU - Sawada, Kazuhisa AU - Liu, Shengao AU - Ozawa, Tadahiro AU - Kodama, Takeko AU - Kakeshita, Hiroshi AU - Kageyama, Yasushi AU - Manabe, Kenji AU - Kanaya, Shigehiko AU - Ara, Katsutoshi AU - Ozaki, Katsuya AU - Ogasawara, Naotake PY - 2008/03/11/ UR - http://dx.doi.org/10.1093/dnares/dsn002 ER -