Crystal structure of a self-spliced group II intron Export

@article{toor08, abstract = {Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a twometal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor. 10.1126/science.1153803}, author = {Toor, Navtej and Keating, Kevin S. and Taylor, Sean D. and Pyle, Anna M.}, citeulike-article-id = {2635699}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1153803}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/320/5872/77}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18388288}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18388288}, day = {4}, doi = {10.1126/science.1153803}, journal = {Science}, keywords = {crystal, groupii, structure}, month = {April}, number = {5872}, pages = {77--82}, posted-at = {2008-04-26 02:15:49}, priority = {0}, title = {Crystal structure of a self-spliced group II intron}, url = {http://dx.doi.org/10.1126/science.1153803}, volume = {320}, year = {2008} }

TY - JOUR ID - toor08 L3 - citeulike-article-id:2635699 N2 - Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a twometal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor. 10.1126/science.1153803 IS - 5872 JF - Science EP - 82 TI - Crystal structure of a self-spliced group II intron VL - 320 SP - 77 KW - crystal KW - groupii KW - structure AU - Toor, Navtej AU - Keating, Kevin AU - Taylor, Sean AU - Pyle, Anna PY - 2008/04/04/ UR - http://dx.doi.org/10.1126/science.1153803 ER -