Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. Export

@article{citeulike:264781, abstract = {Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic "population control" circuit that regulates cell density through a feedback mechanism based on quorum sensing. The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours.}, address = {Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.}, author = {Balagadd\'{e}, Frederick K. and You, Lingchong and Hansen, Carl L. and Arnold, Frances H. and Quake, Stephen R.}, citeulike-article-id = {264781}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1109173}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/309/5731/137}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15994559}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15994559}, day = {1}, doi = {10.1126/science.1109173}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {bioreactor, microfluidics}, month = {July}, number = {5731}, pages = {137--140}, posted-at = {2008-07-12 04:05:45}, priority = {0}, title = {Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.}, url = {http://dx.doi.org/10.1126/science.1109173}, volume = {309}, year = {2005} }

TY - JOUR ID - citeulike:264781 L3 - citeulike-article-id:264781 N2 - Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic "population control" circuit that regulates cell density through a feedback mechanism based on quorum sensing. The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours. IS - 5731 JF - Science (New York, N.Y.) SN - 1095-9203 AD - Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA. EP - 140 TI - Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. VL - 309 SP - 137 KW - bioreactor KW - microfluidics AU - Balagaddé, Frederick AU - You, Lingchong AU - Hansen, Carl AU - Arnold, Frances AU - Quake, Stephen PY - 2005/07/01/ UR - http://dx.doi.org/10.1126/science.1109173 ER -