Modulating the splicing activity of Tetrahymena ribozyme via RNA self-assembly Export

@article{hasegawa06, abstract = {The internal guiding sequence (IGS) is normally located at the 5' end of trans-splicing ribozymes that are derived from the Tetrahymena group I intron, and is required for the recognition of substrate RNAs and for trans-splicing reactions. Here, we separated the Tetrahymena group I intron at the L2 loop to produce two fragments: the IGS-containing substrate, and the IGS-lacking ribozyme. We show here that two fragments can complex not through the IGS interaction but under the guidance of appended interacting nucleotides, and perform trans-splicing. The splicing reactions took place both in vitro and in mammalian cells, and the spliced mRNA product from the self-assembled ribozyme complex can be translated into functional proteins in vivo. The splicing efficiency was dependent on the length of appending nucleotides.}, author = {Hasegawa, Sumitaka and Rao, Jianghong}, citeulike-article-id = {2855292}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.febslet.2006.01.090}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16472807}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16472807}, citeulike-linkout-3 = {http://www.sciencedirect.com/science/article/B6T36-4J62306-7/1/2a9a5d8034911f4a06042ee6c6741ae9}, comment = {split ribozyme in L2 loop, beta-lactamase activity}, day = {6}, doi = {10.1016/j.febslet.2006.01.090}, journal = {FEBS Letters}, keywords = {control, reporter, ribozyme, split, tetrahymena}, month = {March}, number = {6}, pages = {1592--1596}, posted-at = {2008-06-12 22:09:57}, priority = {0}, title = {Modulating the splicing activity of Tetrahymena ribozyme via RNA self-assembly}, url = {http://dx.doi.org/10.1016/j.febslet.2006.01.090}, volume = {580}, year = {2006} }

TY - JOUR ID - hasegawa06 L3 - citeulike-article-id:2855292 N2 - The internal guiding sequence (IGS) is normally located at the 5' end of trans-splicing ribozymes that are derived from the Tetrahymena group I intron, and is required for the recognition of substrate RNAs and for trans-splicing reactions. Here, we separated the Tetrahymena group I intron at the L2 loop to produce two fragments: the IGS-containing substrate, and the IGS-lacking ribozyme. We show here that two fragments can complex not through the IGS interaction but under the guidance of appended interacting nucleotides, and perform trans-splicing. The splicing reactions took place both in vitro and in mammalian cells, and the spliced mRNA product from the self-assembled ribozyme complex can be translated into functional proteins in vivo. The splicing efficiency was dependent on the length of appending nucleotides. IS - 6 JF - FEBS Letters EP - 1596 TI - Modulating the splicing activity of Tetrahymena ribozyme via RNA self-assembly VL - 580 SP - 1592 KW - control KW - reporter KW - ribozyme KW - split KW - tetrahymena N1 - split ribozyme in L2 loop, beta-lactamase activity AU - Hasegawa, Sumitaka AU - Rao, Jianghong PY - 2006/03/06/ UR - http://dx.doi.org/10.1016/j.febslet.2006.01.090 ER -