Aptamers switch on fluorescence of triphenylmethane dyes. Export

@article{babendure03, abstract = {MG and SRB aptamers, which are short RNA sequences originally selected only for binding to malachite green or sulforhodamine B, can greatly enhance the fluorescence of normally nonfluorescent triphenylmethane dyes. MG aptamer enhances the quantum yields of malachite green (MG) and a novel rigidized derivative, indolinyl malachite green (IMG) by >2000-fold. SRB aptamer brightens patent blue V and VF by >90-fold. These enhancements are specific because MG aptamer has no effect on patent blue dyes and SRB aptamer has little or no effect on MG and IMG. Such sequence-specific fluorescence labeling of short RNA motifs is a first step toward genetically encodable fusion tags for imaging selected RNAs in vitro and in cells.}, address = {Department of Pharmacology, Howard Hughes Medical Institute, University of California-San Diego, La Jolla, CA 92093-0647, USA.}, author = {Babendure, J. R. and Adams, S. R. and Tsien, R. Y.}, citeulike-article-id = {2915132}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja037994o}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja037994o}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14640641}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14640641}, day = {3}, doi = {10.1021/ja037994o}, issn = {0002-7863}, journal = {Journal of the American Chemical Society}, keywords = {aptamer, malachitegreen}, month = {December}, number = {48}, pages = {14716--14717}, posted-at = {2008-06-22 14:11:37}, priority = {0}, title = {Aptamers switch on fluorescence of triphenylmethane dyes.}, url = {http://dx.doi.org/10.1021/ja037994o}, volume = {125}, year = {2003} }

TY - JOUR ID - babendure03 L3 - citeulike-article-id:2915132 N2 - MG and SRB aptamers, which are short RNA sequences originally selected only for binding to malachite green or sulforhodamine B, can greatly enhance the fluorescence of normally nonfluorescent triphenylmethane dyes. MG aptamer enhances the quantum yields of malachite green (MG) and a novel rigidized derivative, indolinyl malachite green (IMG) by >2000-fold. SRB aptamer brightens patent blue V and VF by >90-fold. These enhancements are specific because MG aptamer has no effect on patent blue dyes and SRB aptamer has little or no effect on MG and IMG. Such sequence-specific fluorescence labeling of short RNA motifs is a first step toward genetically encodable fusion tags for imaging selected RNAs in vitro and in cells. IS - 48 JF - Journal of the American Chemical Society SN - 0002-7863 AD - Department of Pharmacology, Howard Hughes Medical Institute, University of California-San Diego, La Jolla, CA 92093-0647, USA. EP - 14717 TI - Aptamers switch on fluorescence of triphenylmethane dyes. VL - 125 SP - 14716 KW - aptamer KW - malachitegreen AU - Babendure, JR AU - Adams, SR AU - Tsien, RY PY - 2003/12/03/ UR - http://dx.doi.org/10.1021/ja037994o ER -