Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells. Export

@article{citeulike:3157684, abstract = {In mammalian cells, genetic instructions are usually revised by RNA splicing before they are translated to proteins. Here we demonstrate that a trans-splicing group I ribozyme can be employed to intentionally modify the sequence of targeted transcripts in tissue culture cells. By analyzing the ribozyme reaction products, we demonstrate that targeted trans-splicing can proceed in murine fibroblasts with high fidelity, providing direct evidence that ribozymes function as anticipated in a therapeutically relevant setting. Trans-splicing is not very specific however, and the ribozyme reacted with and tagged a variety of cellular transcripts with its 3' exon sequence. RNA tagging provides a unique approach to study RNA catalysis in mammalian cells. Such analysis should facilitate the logical development of safe, therapeutic ribozymes that can repair mutant RNAs associated with a variety of inherited diseases.}, address = {Department of Experimental Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.}, author = {Jones, J. T. and Lee, S. W. and Sullenger, B. A.}, citeulike-article-id = {3157684}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8640554}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8640554}, issn = {1078-8956}, journal = {Nature medicine}, keywords = {ribozyme, trans-splicing}, month = {June}, number = {6}, pages = {643--648}, posted-at = {2008-08-27 03:53:54}, priority = {0}, title = {Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8640554}, volume = {2}, year = {1996} }

TY - JOUR ID - citeulike:3157684 L3 - citeulike-article-id:3157684 N2 - In mammalian cells, genetic instructions are usually revised by RNA splicing before they are translated to proteins. Here we demonstrate that a trans-splicing group I ribozyme can be employed to intentionally modify the sequence of targeted transcripts in tissue culture cells. By analyzing the ribozyme reaction products, we demonstrate that targeted trans-splicing can proceed in murine fibroblasts with high fidelity, providing direct evidence that ribozymes function as anticipated in a therapeutically relevant setting. Trans-splicing is not very specific however, and the ribozyme reacted with and tagged a variety of cellular transcripts with its 3' exon sequence. RNA tagging provides a unique approach to study RNA catalysis in mammalian cells. Such analysis should facilitate the logical development of safe, therapeutic ribozymes that can repair mutant RNAs associated with a variety of inherited diseases. IS - 6 JF - Nature medicine SN - 1078-8956 AD - Department of Experimental Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA. EP - 648 TI - Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells. VL - 2 SP - 643 KW - ribozyme KW - trans-splicing AU - Jones, JT AU - Lee, SW AU - Sullenger, BA PY - 1996/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/8640554 ER -