Light-up Hoechst-DNA aptamer pair: generation of an aptamer-selective fluorophore from a conventional DNA-staining dye. Export

@article{citeulike:3250761, abstract = {We have designed a strategy to generate a light-up fluorophore-aptamer pair based on a down-modification of a conventional DNA-staining dye to suppress its affinity to the original dsDNA targets, followed by reselection of aptamers that would bind to the modified dye. Following this line, we prepared a micropolarity-sensitive Hoechst derivative possessing two tBu groups with low affinity to the usual AT-rich dsDNA targets. DNA aptamers selected in vitro from a random pool worked as triggers to enhance the fluorescence of an otherwise nonfluorescent Hoechst derivative, and the shortened 25-mer sequence showed remarkable enhancement (light-up). The 25-mer sequence was split into binary aptamer probes, thus enabling us to detect a target nucleic acid sequence with a single-nucleotide resolution by use of unmodified DNA as a probe.}, address = {Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. ssando@sbchem.kyoto-u.ac.jp}, author = {Sando, S. and Narita, A. and Aoyama, Y.}, citeulike-article-id = {3250761}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/cbic.200700325}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17806095}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17806095}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/116312478/ABSTRACT}, day = {15}, doi = {10.1002/cbic.200700325}, issn = {1439-4227}, journal = {Chembiochem : a European journal of chemical biology}, keywords = {aptamer, dna, fluorescent}, month = {October}, number = {15}, pages = {1795--1803}, posted-at = {2008-09-12 17:53:13}, priority = {0}, title = {Light-up Hoechst-DNA aptamer pair: generation of an aptamer-selective fluorophore from a conventional DNA-staining dye.}, url = {http://dx.doi.org/10.1002/cbic.200700325}, volume = {8}, year = {2007} }

TY - JOUR ID - citeulike:3250761 L3 - citeulike-article-id:3250761 N2 - We have designed a strategy to generate a light-up fluorophore-aptamer pair based on a down-modification of a conventional DNA-staining dye to suppress its affinity to the original dsDNA targets, followed by reselection of aptamers that would bind to the modified dye. Following this line, we prepared a micropolarity-sensitive Hoechst derivative possessing two tBu groups with low affinity to the usual AT-rich dsDNA targets. DNA aptamers selected in vitro from a random pool worked as triggers to enhance the fluorescence of an otherwise nonfluorescent Hoechst derivative, and the shortened 25-mer sequence showed remarkable enhancement (light-up). The 25-mer sequence was split into binary aptamer probes, thus enabling us to detect a target nucleic acid sequence with a single-nucleotide resolution by use of unmodified DNA as a probe. IS - 15 JF - Chembiochem : a European journal of chemical biology SN - 1439-4227 AD - Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. ssando@sbchem.kyoto-u.ac.jp EP - 1803 TI - Light-up Hoechst-DNA aptamer pair: generation of an aptamer-selective fluorophore from a conventional DNA-staining dye. VL - 8 SP - 1795 KW - aptamer KW - dna KW - fluorescent AU - Sando, S AU - Narita, A AU - Aoyama, Y PY - 2007/10/15/ UR - http://dx.doi.org/10.1002/cbic.200700325 ER -