One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome Export

@article{citeulike:3877492, abstract = {10.1073/pnas.0811011106 We previously reported assembly and cloning of the synthetic JCVI-1.0 genome in the yeast by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.}, author = {Gibson, Daniel G. and Benders, Gwynedd A. and Axelrod, Kevin C. and Zaveri, Jayshree and Algire, Mikkel A. and Moodie, Monzia and Montague, Michael G. and Venter, Craig J. and Smith, Hamilton O. and Hutchison, Clyde A.}, citeulike-article-id = {3877492}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0811011106}, citeulike-linkout-1 = {http://www.pnas.org/content/105/51/20404.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/51/20404.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19073939}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19073939}, day = {23}, doi = {10.1073/pnas.0811011106}, journal = {Proceedings of the National Academy of Sciences}, keywords = {assembly, recombination}, month = {December}, number = {51}, pages = {20404--20409}, posted-at = {2009-02-20 18:19:35}, priority = {0}, title = {One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome}, url = {http://dx.doi.org/10.1073/pnas.0811011106}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3877492 L3 - citeulike-article-id:3877492 N2 - 10.1073/pnas.0811011106 We previously reported assembly and cloning of the synthetic JCVI-1.0 genome in the yeast by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments. IS - 51 JF - Proceedings of the National Academy of Sciences EP - 20409 TI - One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome VL - 105 SP - 20404 KW - assembly KW - recombination AU - Gibson, Daniel AU - Benders, Gwynedd AU - Axelrod, Kevin AU - Zaveri, Jayshree AU - Algire, Mikkel AU - Moodie, Monzia AU - Montague, Michael AU - Venter, Craig AU - Smith, Hamilton AU - Hutchison, Clyde PY - 2008/12/23/ UR - http://dx.doi.org/10.1073/pnas.0811011106 ER -