Automated unrestricted multigene recombineering for multiprotein complex production Export

@article{citeulike:4525729, abstract = {Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.}, author = {Bieniossek, Christoph and Nie, Yan and Frey, Daniel and Olieric, Natacha and Schaffitzel, Christiane and Collinson, Ian and Romier, Christophe and Berger, Philipp and Richmond, Timothy J. and Steinmetz, Michel O. and Berger, Imre}, citeulike-article-id = {4525729}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nmeth.1326}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nmeth.1326}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19412171}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19412171}, day = {03}, doi = {10.1038/nmeth.1326}, issn = {1548-7091}, journal = {Nature Methods}, keywords = {assembly, dna}, month = {May}, number = {6}, pages = {447--450}, posted-at = {2009-08-06 16:22:26}, priority = {0}, publisher = {Nature Publishing Group}, title = {Automated unrestricted multigene recombineering for multiprotein complex production}, url = {http://dx.doi.org/10.1038/nmeth.1326}, volume = {6}, year = {2009} }

TY - JOUR ID - citeulike:4525729 L3 - citeulike-article-id:4525729 N2 - Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon. IS - 6 JF - Nature Methods SN - 1548-7091 EP - 450 TI - Automated unrestricted multigene recombineering for multiprotein complex production PB - Nature Publishing Group VL - 6 SP - 447 KW - assembly KW - dna AU - Bieniossek, Christoph AU - Nie, Yan AU - Frey, Daniel AU - Olieric, Natacha AU - Schaffitzel, Christiane AU - Collinson, Ian AU - Romier, Christophe AU - Berger, Philipp AU - Richmond, Timothy AU - Steinmetz, Michel AU - Berger, Imre PY - 2009/05/03/ UR - http://dx.doi.org/10.1038/nmeth.1326 ER -