Synthetic Gene Networks That Count Export

@article{citeulike:4677375, abstract = {Synthetic gene networks can be constructed to emulate digital circuits and devices, giving one the ability to program and design cells with some of the principles of modern computing, such as counting. A cellular counter would enable complex synthetic programming and a variety of biotechnology applications. Here, we report two complementary synthetic genetic counters in Escherichia coli that can count up to three induction events: the first, a riboregulated transcriptional cascade, and the second, a recombinase-based cascade of memory units. These modular devices permit counting of varied user-defined inputs over a range of frequencies and can be expanded to count higher numbers. 10.1126/science.1172005}, author = {Friedland, Ari E. and Lu, Timothy K. and Wang, Xiao and Shi, David and Church, George and Collins, James J.}, citeulike-article-id = {4677375}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1172005}, citeulike-linkout-1 = {http://www.sciencemag.org/content/324/5931/1199.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/324/5931/1199.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/324/5931/1199?rss=1}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/19478183}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=19478183}, day = {29}, doi = {10.1126/science.1172005}, journal = {Science}, keywords = {circuit, counter, syntheticbiology}, month = {May}, number = {5931}, pages = {1199--1202}, posted-at = {2009-06-05 22:47:47}, priority = {0}, title = {Synthetic Gene Networks That Count}, url = {http://dx.doi.org/10.1126/science.1172005}, volume = {324}, year = {2009} }

TY - JOUR ID - citeulike:4677375 L3 - citeulike-article-id:4677375 N2 - Synthetic gene networks can be constructed to emulate digital circuits and devices, giving one the ability to program and design cells with some of the principles of modern computing, such as counting. A cellular counter would enable complex synthetic programming and a variety of biotechnology applications. Here, we report two complementary synthetic genetic counters in Escherichia coli that can count up to three induction events: the first, a riboregulated transcriptional cascade, and the second, a recombinase-based cascade of memory units. These modular devices permit counting of varied user-defined inputs over a range of frequencies and can be expanded to count higher numbers. 10.1126/science.1172005 IS - 5931 JF - Science EP - 1202 TI - Synthetic Gene Networks That Count VL - 324 SP - 1199 KW - circuit KW - counter KW - syntheticbiology AU - Friedland, Ari AU - Lu, Timothy AU - Wang, Xiao AU - Shi, David AU - Church, George AU - Collins, James PY - 2009/05/29/ UR - http://dx.doi.org/10.1126/science.1172005 ER -