DNA-catalyzed sequence-specific hydrolysis of DNA. Export

by: Madhavaiah Chandra, Amit Sachdeva, Scott K. Silverman

Nature chemical biology (16 August 2009)

[Posts]

View FullText article

DOI, Pubmed, Hubmed, Rent at DeepDyve

austin's tags for this article

deoxyribozyme

Reviews [Write a review of this article]

Find related articles from these CiteULike users

austin

Find related articles with these CiteULike tags

deoxyribozyme

Abstract

Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.

@article{citeulike:5664681, abstract = {Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.}, author = {Chandra, Madhavaiah and Sachdeva, Amit and Silverman, Scott K.}, citeulike-article-id = {5664681}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nchembio.201}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19684594}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19684594}, day = {16}, doi = {10.1038/nchembio.201}, issn = {1552-4469}, journal = {Nature chemical biology}, keywords = {deoxyribozyme}, month = {August}, posted-at = {2009-08-28 23:02:46}, priority = {0}, title = {DNA-catalyzed sequence-specific hydrolysis of DNA.}, url = {http://dx.doi.org/10.1038/nchembio.201}, year = {2009} }

RIS record

TY - JOUR ID - citeulike:5664681 L3 - citeulike-article-id:5664681 N2 - Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes. JF - Nature chemical biology SN - 1552-4469 TI - DNA-catalyzed sequence-specific hydrolysis of DNA. KW - deoxyribozyme AU - Chandra, Madhavaiah AU - Sachdeva, Amit AU - Silverman, Scott PY - 2009/08/16/ UR - http://dx.doi.org/10.1038/nchembio.201 ER -

DNA-catalyzed sequence-specific hydrolysis of DNA. Export

Citation Format