Towards the uniform distribution of null P values on Affymetrix microarrays Export

@article{citeulike:1271865, abstract = {Methods to control false-positive rates require that P values of genes that are not differentially expressed follow a uniform distribution. Commonly used microarray statistics can generate P values that do not meet this assumption. We show that poorly characterized variance, imperfect normalization, and cross-hybridization are among the many causes of this non-uniform distribution. We demonstrate a simple technique that produces P values that are close to uniform for nondifferentially expressed genes in control datasets.}, author = {Fodor, Anthony and Tickle, Timothy and Richardson, Christine}, citeulike-article-id = {1271865}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/gb-2007-8-5-r69}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17472745}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17472745}, doi = {10.1186/gb-2007-8-5-r69}, issn = {1465-6906}, journal = {Genome Biology}, keywords = {genes}, month = {May}, number = {5}, pages = {R69+}, posted-at = {2009-03-26 02:31:59}, priority = {2}, title = {Towards the uniform distribution of null P values on Affymetrix microarrays}, url = {http://dx.doi.org/10.1186/gb-2007-8-5-r69}, volume = {8}, year = {2007} }

TY - JOUR ID - citeulike:1271865 L3 - citeulike-article-id:1271865 N2 - Methods to control false-positive rates require that P values of genes that are not differentially expressed follow a uniform distribution. Commonly used microarray statistics can generate P values that do not meet this assumption. We show that poorly characterized variance, imperfect normalization, and cross-hybridization are among the many causes of this non-uniform distribution. We demonstrate a simple technique that produces P values that are close to uniform for nondifferentially expressed genes in control datasets. IS - 5 JF - Genome Biology SN - 1465-6906 TI - Towards the uniform distribution of null P values on Affymetrix microarrays VL - 8 SP - R69 KW - genes AU - Fodor, Anthony AU - Tickle, Timothy AU - Richardson, Christine PY - 2007/05/01/ UR - http://dx.doi.org/10.1186/gb-2007-8-5-r69 ER -