Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage. Export

@article{citeulike:2394541, abstract = {In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.}, address = {Department of Microbiology, Korean Institute of Tuberculosis, Seoul, Republic of Korea.}, author = {Ryoo, S. W. and Park, Y. K. and Park, S. N. and Shim, Y. S. and Liew, H. and Kang, S. and Bai, G. H.}, citeulike-article-id = {2394541}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/17618234}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=17618234}, issn = {1225-8873}, journal = {J Microbiol}, keywords = {glycigen, phosphorylase}, month = {June}, number = {3}, pages = {268--271}, posted-at = {2008-02-18 14:08:54}, priority = {2}, title = {Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17618234}, volume = {45}, year = {2007} }

TY - JOUR ID - citeulike:2394541 L3 - citeulike-article-id:2394541 N2 - In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis. IS - 3 JF - J Microbiol SN - 1225-8873 AD - Department of Microbiology, Korean Institute of Tuberculosis, Seoul, Republic of Korea. EP - 271 TI - Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage. VL - 45 SP - 268 KW - glycigen KW - phosphorylase AU - Ryoo, SW AU - Park, YK AU - Park, SN AU - Shim, YS AU - Liew, H AU - Kang, S AU - Bai, GH PY - 2007/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/17618234 ER -