The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation. Export

@article{citeulike:2709904, abstract = {Pseudomonas aeruginosa, an important opportunistic human pathogen, persists in certain tissues in the form of specialized bacterial communities, referred to as biofilm. The biofilm is formed through series of interactions between cells and adherence to surfaces, resulting in an organized structure. By screening a library of Tn5 insertions in a nonpiliated P. aeruginosa strain, we identified genes involved in early stages of biofilm formation. One class of mutations identified in this study mapped in a cluster of genes specifying the components of a chaperone/usher pathway that is involved in assembly of fimbrial subunits in other microorganisms. These genes, not previously described in P. aeruginosa, were named cupA1-A5. Additional chaperone/usher systems (CupB and CupC) have been also identified in the genome of P. aeruginosa PAO1; however, they do not appear to play a role in adhesion under the conditions where the CupA system is expressed and functions in surface adherence. The identification of these putative adhesins on the cell surface of P. aeruginosa suggests that this organism possess a wide range of factors that function in biofilm formation. These structures appear to be differentially regulated and may function at distinct stages of biofilm formation, or in specific environments colonized by this organism.}, address = {Laboratoire d'Ing\'{e}ni\'{e}rie des Syst\`{e}mes Macromol\'{e}culaires, Unit\'{e} Propre de Recherche 9027, Institut de Biologie Structurale et Microbiologie/Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.}, author = {Vallet, I. and Olson, J. W. and Lory, S. and Lazdunski, A. and Filloux, A.}, citeulike-article-id = {2709904}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.111551898}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11381121}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11381121}, day = {5}, doi = {10.1073/pnas.111551898}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {aeruginosa, biofilm, cupa}, month = {June}, number = {12}, pages = {6911--6916}, posted-at = {2008-04-23 21:03:30}, priority = {2}, title = {The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation.}, url = {http://dx.doi.org/10.1073/pnas.111551898}, volume = {98}, year = {2001} }

TY - JOUR ID - citeulike:2709904 L3 - citeulike-article-id:2709904 N2 - Pseudomonas aeruginosa, an important opportunistic human pathogen, persists in certain tissues in the form of specialized bacterial communities, referred to as biofilm. The biofilm is formed through series of interactions between cells and adherence to surfaces, resulting in an organized structure. By screening a library of Tn5 insertions in a nonpiliated P. aeruginosa strain, we identified genes involved in early stages of biofilm formation. One class of mutations identified in this study mapped in a cluster of genes specifying the components of a chaperone/usher pathway that is involved in assembly of fimbrial subunits in other microorganisms. These genes, not previously described in P. aeruginosa, were named cupA1-A5. Additional chaperone/usher systems (CupB and CupC) have been also identified in the genome of P. aeruginosa PAO1; however, they do not appear to play a role in adhesion under the conditions where the CupA system is expressed and functions in surface adherence. The identification of these putative adhesins on the cell surface of P. aeruginosa suggests that this organism possess a wide range of factors that function in biofilm formation. These structures appear to be differentially regulated and may function at distinct stages of biofilm formation, or in specific environments colonized by this organism. IS - 12 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Laboratoire d'Ingéniérie des Systèmes Macromoléculaires, Unité Propre de Recherche 9027, Institut de Biologie Structurale et Microbiologie/Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. EP - 6916 TI - The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation. VL - 98 SP - 6911 KW - aeruginosa KW - biofilm KW - cupa AU - Vallet, I AU - Olson, JW AU - Lory, S AU - Lazdunski, A AU - Filloux, A PY - 2001/06/05/ UR - http://dx.doi.org/10.1073/pnas.111551898 ER -