The Influence of Iron on Pseudomonas aeruginosa Physiology: A REGULATORY LINK BETWEEN IRON AND QUORUM SENSING Export

@article{citeulike:2891533, abstract = {In iron-replete environments, the Pseudomonas aeruginosa Fur (ferric uptake regulator) protein represses expression of two small regulatory RNAs encoded by prrF1 and prrF2. Here we describe the effects of iron and PrrF regulation on P. aeruginosa physiology. We show that PrrF represses genes encoding enzymes for the degradation of anthranilate (i.e. antABC), a precursor of the Pseudomonas quinolone signal (PQS). Under iron-limiting conditions, PQS production was greatly decreased in a {Delta}prrF1,2 mutant as compared with wild type. The addition of anthranilate to the growth medium restored PQS production to the {Delta}prrF1,2 mutant, indicating that its defect in PQS production is a consequence of anthranilate degradation. PA2511 was shown to encode an anthranilate-dependent activator of the ant genes and was subsequently renamed antR. AntR was not required for regulation of antA by PrrF but was required for optimal iron activation of antA. Furthermore, iron was capable of activating both antA and antR in a {Delta}prrF1,2 mutant, indicating the presence of two distinct yet overlapping pathways for iron activation of antA (AntR-dependent and PrrF-dependent). Additionally, several quorum-sensing regulators, including PqsR, influenced antA expression, demonstrating that regulation of anthranilate metabolism is intimately woven into the quorum-sensing network of P. aeruginosa. Overall, our data illustrate the extensive control that both iron regulation and quorum sensing exercise in basic cellular physiology, underlining how intermediary metabolism can affect the regulation of virulence factors in P. aeruginosa. 10.1074/jbc.M707840200}, author = {Oglesby, Amanda G. and Farrow, John M. and Lee, Joon-Hee and Tomaras, Andrew P. and Greenberg, E. P. and Pesci, Everett C. and Vasil, Michael L.}, citeulike-article-id = {2891533}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M707840200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/283/23/15558}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18424436}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18424436}, day = {6}, doi = {10.1074/jbc.M707840200}, journal = {J. Biol. Chem.}, keywords = {aeruginosa, iron, prrf}, month = {June}, number = {23}, pages = {15558--15567}, posted-at = {2008-07-28 01:16:31}, priority = {2}, title = {The Influence of Iron on Pseudomonas aeruginosa Physiology: A REGULATORY LINK BETWEEN IRON AND QUORUM SENSING}, url = {http://dx.doi.org/10.1074/jbc.M707840200}, volume = {283}, year = {2008} }

TY - JOUR ID - citeulike:2891533 L3 - citeulike-article-id:2891533 N2 - In iron-replete environments, the Pseudomonas aeruginosa Fur (ferric uptake regulator) protein represses expression of two small regulatory RNAs encoded by prrF1 and prrF2. Here we describe the effects of iron and PrrF regulation on P. aeruginosa physiology. We show that PrrF represses genes encoding enzymes for the degradation of anthranilate (i.e. antABC), a precursor of the Pseudomonas quinolone signal (PQS). Under iron-limiting conditions, PQS production was greatly decreased in a {Delta}prrF1,2 mutant as compared with wild type. The addition of anthranilate to the growth medium restored PQS production to the {Delta}prrF1,2 mutant, indicating that its defect in PQS production is a consequence of anthranilate degradation. PA2511 was shown to encode an anthranilate-dependent activator of the ant genes and was subsequently renamed antR. AntR was not required for regulation of antA by PrrF but was required for optimal iron activation of antA. Furthermore, iron was capable of activating both antA and antR in a {Delta}prrF1,2 mutant, indicating the presence of two distinct yet overlapping pathways for iron activation of antA (AntR-dependent and PrrF-dependent). Additionally, several quorum-sensing regulators, including PqsR, influenced antA expression, demonstrating that regulation of anthranilate metabolism is intimately woven into the quorum-sensing network of P. aeruginosa. Overall, our data illustrate the extensive control that both iron regulation and quorum sensing exercise in basic cellular physiology, underlining how intermediary metabolism can affect the regulation of virulence factors in P. aeruginosa. 10.1074/jbc.M707840200 IS - 23 JF - J. Biol. Chem. EP - 15567 TI - The Influence of Iron on Pseudomonas aeruginosa Physiology: A REGULATORY LINK BETWEEN IRON AND QUORUM SENSING VL - 283 SP - 15558 KW - aeruginosa KW - iron KW - prrf AU - Oglesby, Amanda AU - Farrow, John AU - Lee, Joon-Hee AU - Tomaras, Andrew AU - Greenberg, EP AU - Pesci, Everett AU - Vasil, Michael PY - 2008/06/06/ UR - http://dx.doi.org/10.1074/jbc.M707840200 ER -