Pseudomonas aeruginosa Virulence Analyzed in a Dictyostelium discoideum Host System Export

@article{citeulike:2987429, abstract = {Pseudomonas aeruginosa is an important opportunistic pathogen that produces a variety of cell-associated and secreted virulence factors. P. aeruginosa infections are difficult to treat effectively because of the rapid emergence of antibiotic-resistant strains. In this study, we analyzed whether the amoeba Dictyostelium discoideum can be used as a simple model system to analyze the virulence of P. aeruginosa strains. The virulent wild-type strain PAO1 was shown to inhibit growth of D. discoideum. Isogenic mutants deficient in the las quorum-sensing system were almost as inhibitory as the wild type, while rhl quorum-sensing mutants permitted growth of Dictyostelium cells. Therefore, in this model system, factors controlled by the rhl quorum-sensing system were found to play a central role. Among these, rhamnolipids secreted by the wild-type strain PAO1 could induce fast lysis of D. discoideum cells. By using this simple model system, we predicted that certain antibiotic-resistant mutants of P. aeruginosa should show reduced virulence. This result was confirmed in a rat model of acute pneumonia. Thus, D. discoideum could be used as a simple nonmammalian host system to assess pathogenicity of P. aeruginosa. 10.1128/JB.184.11.3027-3033.2002}, author = {Cosson, Pierre and Zulianello, Laurence and Join-Lambert, Olivier and Faurisson, Francois and Gebbie, Leigh and Benghezal, Mohammed and van Delden, Christian and Curty, Lasta K. and Kohler, Thilo}, citeulike-article-id = {2987429}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JB.184.11.3027-3033.2002}, citeulike-linkout-1 = {http://jb.asm.org/cgi/content/abstract/184/11/3027}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12003944}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12003944}, day = {1}, doi = {10.1128/JB.184.11.3027-3033.2002}, journal = {J. Bacteriol.}, keywords = {aeruginosa, model}, month = {June}, number = {11}, pages = {3027--3033}, posted-at = {2008-07-11 10:49:45}, priority = {2}, title = {Pseudomonas aeruginosa Virulence Analyzed in a Dictyostelium discoideum Host System}, url = {http://dx.doi.org/10.1128/JB.184.11.3027-3033.2002}, volume = {184}, year = {2002} }

TY - JOUR ID - citeulike:2987429 L3 - citeulike-article-id:2987429 N2 - Pseudomonas aeruginosa is an important opportunistic pathogen that produces a variety of cell-associated and secreted virulence factors. P. aeruginosa infections are difficult to treat effectively because of the rapid emergence of antibiotic-resistant strains. In this study, we analyzed whether the amoeba Dictyostelium discoideum can be used as a simple model system to analyze the virulence of P. aeruginosa strains. The virulent wild-type strain PAO1 was shown to inhibit growth of D. discoideum. Isogenic mutants deficient in the las quorum-sensing system were almost as inhibitory as the wild type, while rhl quorum-sensing mutants permitted growth of Dictyostelium cells. Therefore, in this model system, factors controlled by the rhl quorum-sensing system were found to play a central role. Among these, rhamnolipids secreted by the wild-type strain PAO1 could induce fast lysis of D. discoideum cells. By using this simple model system, we predicted that certain antibiotic-resistant mutants of P. aeruginosa should show reduced virulence. This result was confirmed in a rat model of acute pneumonia. Thus, D. discoideum could be used as a simple nonmammalian host system to assess pathogenicity of P. aeruginosa. 10.1128/JB.184.11.3027-3033.2002 IS - 11 JF - J. Bacteriol. EP - 3033 TI - Pseudomonas aeruginosa Virulence Analyzed in a Dictyostelium discoideum Host System VL - 184 SP - 3027 KW - aeruginosa KW - model AU - Cosson, Pierre AU - Zulianello, Laurence AU - Join-Lambert, Olivier AU - Faurisson, Francois AU - Gebbie, Leigh AU - Benghezal, Mohammed AU - van Delden, Christian AU - Curty, Lasta AU - Kohler, Thilo PY - 2002/06/01/ UR - http://dx.doi.org/10.1128/JB.184.11.3027-3033.2002 ER -