Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Export

@article{citeulike:2987597, abstract = {In many human infections, hosts and pathogens coexist for years or decades. Important examples include HIV, herpes viruses, tuberculosis, leprosy, and malaria. With the exception of intensively studied viral infections such as HIV/AIDs, little is known about the extent to which the clonal expansion that occurs during long-term infection by pathogens involves important genetic adaptations. We report here a detailed, whole-genome analysis of one such infection, that of a cystic fibrosis (CF) patient by the opportunistic bacterial pathogen Pseudomonas aeruginosa. The bacteria underwent numerous genetic adaptations during 8 years of infection, as evidenced by a positive-selection signal across the genome and an overwhelming signal in specific genes, several of which are mutated during the course of most CF infections. Of particular interest is our finding that virulence factors that are required for the initiation of acute infections are often selected against during chronic infections. It is apparent that the genotypes of the P. aeruginosa strains present in advanced CF infections differ systematically from those of "wild-type" P. aeruginosa and that these differences may offer new opportunities for treatment of this chronic disease.}, address = {Genome Center, Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA. smithee@u.washington.edu}, author = {Smith, E. E. and Buckley, D. G. and Wu, Z. and Saenphimmachak, C. and Hoffman, L. R. and D'Argenio, D. A. and Miller, S. I. and Ramsey, B. W. and Speert, D. P. and Moskowitz, S. M. and Burns, J. L. and Kaul, R. and Olson, M. V.}, citeulike-article-id = {2987597}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0602138103}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16687478}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16687478}, day = {30}, doi = {10.1073/pnas.0602138103}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {aeruginosa, cf, genom}, month = {May}, number = {22}, pages = {8487--8492}, posted-at = {2008-07-11 11:00:46}, priority = {2}, title = {Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients.}, url = {http://dx.doi.org/10.1073/pnas.0602138103}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:2987597 L3 - citeulike-article-id:2987597 N2 - In many human infections, hosts and pathogens coexist for years or decades. Important examples include HIV, herpes viruses, tuberculosis, leprosy, and malaria. With the exception of intensively studied viral infections such as HIV/AIDs, little is known about the extent to which the clonal expansion that occurs during long-term infection by pathogens involves important genetic adaptations. We report here a detailed, whole-genome analysis of one such infection, that of a cystic fibrosis (CF) patient by the opportunistic bacterial pathogen Pseudomonas aeruginosa. The bacteria underwent numerous genetic adaptations during 8 years of infection, as evidenced by a positive-selection signal across the genome and an overwhelming signal in specific genes, several of which are mutated during the course of most CF infections. Of particular interest is our finding that virulence factors that are required for the initiation of acute infections are often selected against during chronic infections. It is apparent that the genotypes of the P. aeruginosa strains present in advanced CF infections differ systematically from those of "wild-type" P. aeruginosa and that these differences may offer new opportunities for treatment of this chronic disease. IS - 22 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Genome Center, Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA. smithee@u.washington.edu EP - 8492 TI - Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. VL - 103 SP - 8487 KW - aeruginosa KW - cf KW - genom AU - Smith, EE AU - Buckley, DG AU - Wu, Z AU - Saenphimmachak, C AU - Hoffman, LR AU - D'Argenio, DA AU - Miller, SI AU - Ramsey, BW AU - Speert, DP AU - Moskowitz, SM AU - Burns, JL AU - Kaul, R AU - Olson, MV PY - 2006/05/30/ UR - http://dx.doi.org/10.1073/pnas.0602138103 ER -