Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound Export

@article{citeulike:2988119, abstract = {Novel molecular tools have been constructed which allow for in situ detection of N-acyl homoserine lactone (AHL)-mediated quorum sensing in Pseudomonas aeruginosa biofilms. The reporter responds to AHL activation of LasR by expression of an unstable version of the green-fluorescent protein (Gfp). Gfp-based reporter technology has been applied for non-destructive, single-cell-level detection of quorum sensing in laboratory-based P. aeruginosa biofilms. It is reported that a synthetic halogenated furanone compound, which is a derivative of the secondary metabolites produced by the Australian macroalga Delisea pulchra, is capable of interfering with AHL-mediated quorum sensing in P. aeruginosa. It is demonstrated that the furanone compound specifically represses expression of a PlasB-gfp reporter fusion without affecting growth or protein synthesis. In addition, it reduces the production of important virulence factors, indicating a general effect on target genes of the las quorum sensing circuit. The furanone was applied to P. aeruginosa biofilms established in biofilm flow chambers. The Gfp-based analysis reveals that the compound penetrates microcolonies and blocks cell signalling and quorum sensing in most biofilm cells. The compound did not affect initial attachment to the abiotic substratum. It does, however, affect the architecture of the biofilm and enhances the process of bacterial detachment, leading to a loss of bacterial biomass from the substratum.}, author = {Hentzer, Morten and Riedel, Kathrin and Rasmussen, Thomas B. and Heydorn, Arne and Andersen, Jens B. and Parsek, Matthew R. and Rice, Scott A. and Eberl, Leo and Molin, Soren and Hoiby, Niels and Kjelleberg, Staffan and Givskov, Michael}, citeulike-article-id = {2988119}, citeulike-linkout-0 = {http://mic.sgmjournals.org/cgi/content/abstract/148/1/87}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11782502}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11782502}, day = {1}, journal = {Microbiology}, keywords = {aeruginosa, biofilm, gfp, qs}, month = {January}, number = {1}, pages = {87--102}, posted-at = {2008-07-11 13:28:05}, priority = {2}, title = {Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound}, url = {http://mic.sgmjournals.org/cgi/content/abstract/148/1/87}, volume = {148}, year = {2002} }

TY - JOUR ID - citeulike:2988119 L3 - citeulike-article-id:2988119 N2 - Novel molecular tools have been constructed which allow for in situ detection of N-acyl homoserine lactone (AHL)-mediated quorum sensing in Pseudomonas aeruginosa biofilms. The reporter responds to AHL activation of LasR by expression of an unstable version of the green-fluorescent protein (Gfp). Gfp-based reporter technology has been applied for non-destructive, single-cell-level detection of quorum sensing in laboratory-based P. aeruginosa biofilms. It is reported that a synthetic halogenated furanone compound, which is a derivative of the secondary metabolites produced by the Australian macroalga Delisea pulchra, is capable of interfering with AHL-mediated quorum sensing in P. aeruginosa. It is demonstrated that the furanone compound specifically represses expression of a PlasB-gfp reporter fusion without affecting growth or protein synthesis. In addition, it reduces the production of important virulence factors, indicating a general effect on target genes of the las quorum sensing circuit. The furanone was applied to P. aeruginosa biofilms established in biofilm flow chambers. The Gfp-based analysis reveals that the compound penetrates microcolonies and blocks cell signalling and quorum sensing in most biofilm cells. The compound did not affect initial attachment to the abiotic substratum. It does, however, affect the architecture of the biofilm and enhances the process of bacterial detachment, leading to a loss of bacterial biomass from the substratum. IS - 1 JF - Microbiology EP - 102 TI - Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound VL - 148 SP - 87 KW - aeruginosa KW - biofilm KW - gfp KW - qs AU - Hentzer, Morten AU - Riedel, Kathrin AU - Rasmussen, Thomas AU - Heydorn, Arne AU - Andersen, Jens AU - Parsek, Matthew AU - Rice, Scott AU - Eberl, Leo AU - Molin, Soren AU - Hoiby, Niels AU - Kjelleberg, Staffan AU - Givskov, Michael PY - 2002/01/01/ UR - http://mic.sgmjournals.org/cgi/content/abstract/148/1/87 ER -