Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. Export

@article{citeulike:2997278, abstract = {The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini-Tn5 derivatives has been constructed. The potential of coupling mini-Tn5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated.}, address = {Food Microbiology Section, School of Biological Sciences, University of Nottingham Leics, USA.}, author = {Winson, M. K. and Swift, S. and Hill, P. J. and Sims, C. M. and Griesmayr, G. and Bycroft, B. W. and Williams, P. and Stewart, G. S.}, citeulike-article-id = {2997278}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9673022}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9673022}, day = {15}, issn = {0378-1097}, journal = {FEMS microbiology letters}, keywords = {lux}, month = {June}, number = {2}, pages = {193--202}, posted-at = {2008-07-13 14:29:23}, priority = {2}, title = {Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9673022}, volume = {163}, year = {1998} }

TY - JOUR ID - citeulike:2997278 L3 - citeulike-article-id:2997278 N2 - The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini-Tn5 derivatives has been constructed. The potential of coupling mini-Tn5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated. IS - 2 JF - FEMS microbiology letters SN - 0378-1097 AD - Food Microbiology Section, School of Biological Sciences, University of Nottingham Leics, USA. EP - 202 TI - Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. VL - 163 SP - 193 KW - lux AU - Winson, MK AU - Swift, S AU - Hill, PJ AU - Sims, CM AU - Griesmayr, G AU - Bycroft, BW AU - Williams, P AU - Stewart, GS PY - 1998/06/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9673022 ER -