Rapid, Nongenomic Responses to Ecdysteroids and Catecholamines Mediated by a Novel Drosophila G-Protein-Coupled Receptor Export

@article{citeulike:4574831, abstract = {Nongenomic response pathways mediate many of the rapid actions of steroid hormones, but the mechanisms underlying such responses remain controversial. In some cases, cell-surface expression of classical nuclear steroid receptors has been suggested to mediate these effects, but, in a few instances, specific G-protein-coupled receptors (GPCRs) have been reported to be responsible. Here, we describe the activation of a novel, neuronally expressed Drosophila GPCR by the insect ecdysteroids ecdysone (E) and 20-hydroxyecdysone (20E). This is the first report of an identified insect GPCR interacting with steroids. The Drosophila melanogaster dopamine/ecdysteroid receptor (DmDopEcR) shows sequence homology with vertebrate {beta}-adrenergic receptors and is activated by dopamine (DA) to increase cAMP levels and to activate the phosphoinositide 3-kinase pathway. Conversely, E and 20E show high affinity for the receptor in binding studies and can inhibit the effects of DA, as well as coupling the receptor to a rapid activation of the mitogen-activated protein kinase pathway. The receptor may thus represent the Drosophila homolog of the vertebrate "{gamma}-adrenergic receptors," which are responsible for the modulation of various activities in brain, blood vessels, and pancreas. Thus, DmDopEcR can function as a cell-surface GPCR that may be responsible for some of the rapid, nongenomic actions of ecdysteroids, during both development and signaling in the mature adult nervous system. 10.1523/JNEUROSCI.1005-05.2005}, author = {Srivastava, Deepak P. and Yu, Esther J. and Kennedy, Karen and Chatwin, Heather and Reale, Vincenzina and Hamon, Maureen and Smith, Trevor and Evans, Peter D.}, citeulike-article-id = {4574831}, citeulike-linkout-0 = {http://dx.doi.org/10.1523/JNEUROSCI.1005-05.2005}, citeulike-linkout-1 = {http://www.jneurosci.org/cgi/content/abstract/25/26/6145}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15987944}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15987944}, day = {29}, doi = {10.1523/JNEUROSCI.1005-05.2005}, journal = {J. Neurosci.}, keywords = {catecholamines, dopamine, drosophila, gpcr}, month = {June}, number = {26}, pages = {6145--6155}, posted-at = {2009-05-22 03:08:22}, priority = {2}, title = {Rapid, Nongenomic Responses to Ecdysteroids and Catecholamines Mediated by a Novel Drosophila G-Protein-Coupled Receptor}, url = {http://dx.doi.org/10.1523/JNEUROSCI.1005-05.2005}, volume = {25}, year = {2005} }

TY - JOUR ID - citeulike:4574831 L3 - citeulike-article-id:4574831 N2 - Nongenomic response pathways mediate many of the rapid actions of steroid hormones, but the mechanisms underlying such responses remain controversial. In some cases, cell-surface expression of classical nuclear steroid receptors has been suggested to mediate these effects, but, in a few instances, specific G-protein-coupled receptors (GPCRs) have been reported to be responsible. Here, we describe the activation of a novel, neuronally expressed Drosophila GPCR by the insect ecdysteroids ecdysone (E) and 20-hydroxyecdysone (20E). This is the first report of an identified insect GPCR interacting with steroids. The Drosophila melanogaster dopamine/ecdysteroid receptor (DmDopEcR) shows sequence homology with vertebrate {beta}-adrenergic receptors and is activated by dopamine (DA) to increase cAMP levels and to activate the phosphoinositide 3-kinase pathway. Conversely, E and 20E show high affinity for the receptor in binding studies and can inhibit the effects of DA, as well as coupling the receptor to a rapid activation of the mitogen-activated protein kinase pathway. The receptor may thus represent the Drosophila homolog of the vertebrate "{gamma}-adrenergic receptors," which are responsible for the modulation of various activities in brain, blood vessels, and pancreas. Thus, DmDopEcR can function as a cell-surface GPCR that may be responsible for some of the rapid, nongenomic actions of ecdysteroids, during both development and signaling in the mature adult nervous system. 10.1523/JNEUROSCI.1005-05.2005 IS - 26 JF - J. Neurosci. EP - 6155 TI - Rapid, Nongenomic Responses to Ecdysteroids and Catecholamines Mediated by a Novel Drosophila G-Protein-Coupled Receptor VL - 25 SP - 6145 KW - catecholamines KW - dopamine KW - drosophila KW - gpcr AU - Srivastava, Deepak AU - Yu, Esther AU - Kennedy, Karen AU - Chatwin, Heather AU - Reale, Vincenzina AU - Hamon, Maureen AU - Smith, Trevor AU - Evans, Peter PY - 2005/06/29/ UR - http://dx.doi.org/10.1523/JNEUROSCI.1005-05.2005 ER -