Fluorescent False Neurotransmitters Visualize Dopamine Release from Individual Presynaptic Terminals Export

@article{citeulike:4702863, abstract = {The nervous system transmits signals between neurons via neurotransmitter release during synaptic vesicle fusion. In order to observe neurotransmitter uptake and release from individual presynaptic terminals directly, we designed fluorescent false neurotransmitters as substrates for the synaptic vesicle monoamine transporter. Using these probes to image dopamine release in the striatum, we made several observations pertinent to synaptic plasticity. We found that the fraction of synaptic vesicles releasing neurotransmitter per stimulus was dependent on the stimulus frequency. A kinetically distinct "reserve" synaptic vesicle population was not observed under these experimental conditions. A frequency-dependent heterogeneity of presynaptic terminals was revealed that was dependent in part on D2 dopamine receptors, indicating a mechanism for frequency-dependent coding of presynaptic selection. 10.1126/science.1172278}, author = {Gubernator, Niko G. G. and Zhang, Hui and Staal, Roland G. W. G. and Mosharov, Eugene V. V. and Pereira, Daniela and Yue, Minerva and Balsanek, Vojtech and Vadola, Paul A. A. and Mukherjee, Bipasha and Edwards, Robert H. H. and Sulzer, David and Sames, Dalibor}, citeulike-article-id = {4702863}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1172278}, citeulike-linkout-1 = {http://www.f1000.com/article/id/1160555}, citeulike-linkout-2 = {http://www.sciencemag.org/cgi/content/abstract/324/5933/1441?sa_campaign=Email}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19423778}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19423778}, day = {12}, doi = {10.1126/science.1172278}, issn = {1095-9203}, journal = {Science}, keywords = {dopamine, imaging, method}, month = {June}, number = {5933}, pages = {1441--1444}, posted-at = {2009-06-16 04:13:28}, priority = {2}, title = {Fluorescent False Neurotransmitters Visualize Dopamine Release from Individual Presynaptic Terminals}, url = {http://dx.doi.org/10.1126/science.1172278}, volume = {324}, year = {2009} }

TY - JOUR ID - citeulike:4702863 L3 - citeulike-article-id:4702863 N2 - The nervous system transmits signals between neurons via neurotransmitter release during synaptic vesicle fusion. In order to observe neurotransmitter uptake and release from individual presynaptic terminals directly, we designed fluorescent false neurotransmitters as substrates for the synaptic vesicle monoamine transporter. Using these probes to image dopamine release in the striatum, we made several observations pertinent to synaptic plasticity. We found that the fraction of synaptic vesicles releasing neurotransmitter per stimulus was dependent on the stimulus frequency. A kinetically distinct "reserve" synaptic vesicle population was not observed under these experimental conditions. A frequency-dependent heterogeneity of presynaptic terminals was revealed that was dependent in part on D2 dopamine receptors, indicating a mechanism for frequency-dependent coding of presynaptic selection. 10.1126/science.1172278 IS - 5933 JF - Science SN - 1095-9203 EP - 1444 TI - Fluorescent False Neurotransmitters Visualize Dopamine Release from Individual Presynaptic Terminals VL - 324 SP - 1441 KW - dopamine KW - imaging KW - method AU - Gubernator, Niko AU - Zhang, Hui AU - Staal, Roland AU - Mosharov, Eugene AU - Pereira, Daniela AU - Yue, Minerva AU - Balsanek, Vojtech AU - Vadola, Paul AU - Mukherjee, Bipasha AU - Edwards, Robert AU - Sulzer, David AU - Sames, Dalibor PY - 2009/06/12/ UR - http://dx.doi.org/10.1126/science.1172278 ER -