A plant-produced plague vaccine candidate confers protection to monkeys Export

@article{citeulike:2152459, abstract = {Production of vaccine antigens in plants has received considerable attention over the last decade. However, despite many antigens being expressed in plant systems, and promising efficacy data with rodent models, few vaccine candidates have advanced into studies in non-human primates or human clinical trials. Here, we report on the transient expression of the F1 and LcrV antigens of Yersinia pestis in Nicotiana benthamiana. The antigens were expressed as fusions to the thermostable enzyme of Clostridium thermocellum. When administered to Cynomolgus Macaques the purified plant-produced antigens induced serum IgG and IgA responses specific to F1 and LcrV, and conferred complete protection against lethal challenge with Y. pestis. This study clearly demonstrates the efficacy of a plant-produced plague vaccine candidate in a primate model.}, author = {Mett, Vadim and Lyons, Jarred and Musiychuk, Konstantin and Chichester, Jessica A. and Brasil, Trevor and Couch, Ronald and Sherwood, Robert and Palmer, Gene A. and Streatfield, Stephen J. and Yusibov, Vidadi}, booktitle = {Vaccines, Immunisation and Immunotherapy - Based on the Fifth World Congress on Vaccines, Immunisation and Immunotherapy}, citeulike-article-id = {2152459}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.vaccine.2007.01.017}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17287055}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17287055}, citeulike-linkout-3 = {http://www.sciencedirect.com/science/article/B6TD4-4MW8WKS-2/2/6f9018cfefb082d134f64d8774f5573a}, day = {20}, doi = {10.1016/j.vaccine.2007.01.017}, journal = {Vaccine}, keywords = {benthamiana, plague, transient}, month = {April}, number = {16}, pages = {3014--3017}, posted-at = {2007-12-20 17:12:32}, priority = {4}, title = {A plant-produced plague vaccine candidate confers protection to monkeys}, url = {http://dx.doi.org/10.1016/j.vaccine.2007.01.017}, volume = {25}, year = {2007} }

TY - JOUR ID - citeulike:2152459 L3 - citeulike-article-id:2152459 N2 - Production of vaccine antigens in plants has received considerable attention over the last decade. However, despite many antigens being expressed in plant systems, and promising efficacy data with rodent models, few vaccine candidates have advanced into studies in non-human primates or human clinical trials. Here, we report on the transient expression of the F1 and LcrV antigens of Yersinia pestis in Nicotiana benthamiana. The antigens were expressed as fusions to the thermostable enzyme of Clostridium thermocellum. When administered to Cynomolgus Macaques the purified plant-produced antigens induced serum IgG and IgA responses specific to F1 and LcrV, and conferred complete protection against lethal challenge with Y. pestis. This study clearly demonstrates the efficacy of a plant-produced plague vaccine candidate in a primate model. IS - 16 JF - Vaccine EP - 3017 TI - A plant-produced plague vaccine candidate confers protection to monkeys VL - 25 T2 - Vaccines, Immunisation and Immunotherapy - Based on the Fifth World Congress on Vaccines, Immunisation and Immunotherapy SP - 3014 KW - benthamiana KW - plague KW - transient AU - Mett, Vadim AU - Lyons, Jarred AU - Musiychuk, Konstantin AU - Chichester, Jessica AU - Brasil, Trevor AU - Couch, Ronald AU - Sherwood, Robert AU - Palmer, Gene AU - Streatfield, Stephen AU - Yusibov, Vidadi PY - 2007/04/20/ UR - http://dx.doi.org/10.1016/j.vaccine.2007.01.017 ER -