Barley Lunasin Suppresses ras-Induced Colony Formation and Inhibits Core Histone Acetylation in Mammalian Cells Export

@article{citeulike:2649745, abstract = {Abstract: Lunasin is a novel peptide originally identified in soybean that suppresses chemical carcinogen-induced transformation in mammalian cells and skin carcinogenesis in mice. Since the lunasin gene was cloned from soybean and the chemically synthesized form of the lunasin peptide has been used in experiments conducted so far, the isolation of lunasin from other natural sources and testing of its biological properties have not been carried out. We report here the isolation, purification, and biological assay of lunasin from barley, a newly found rich source of the peptide. The identity of lunasin was established by Western blot analysis and mass spectrometric peptide mapping of the in-gel tryptic digest of the putative protein band. Lunasin was partially purified with anion exchange and immunoaffinity chromatography. The crude and partially purified lunasin from barley suppressed colony formation in stably ras-transfected mouse fibroblast cells induced with IPTG. These fractions also inhibited histone acetylation in mouse fibroblast NIH 3T3 and human breast MCF-7 cells in the presence of the histone deacetylase inhibitor sodium butyrate. Keywords: Barley lunasin; chemopreventive peptide; isolation; purification and bioassay; mass spectrometric peptide mapping; suppression of colony formation; ras oncogene; inhibition of histone acetylation}, address = {School of Bioresources, University of Andong, Kyungpook, Korea, BioRad Laboratories, Inc., Hercules, California, and Department of Nutritional Sciences \& Toxicology, University of California, Berkeley, California 94720}, author = {Jeong, H. J. and Lam, Y. and de Lumen, B. O.}, citeulike-article-id = {2649745}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/jf0256945}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/jf0256945}, day = {9}, doi = {10.1021/jf0256945}, journal = {J. Agric. Food Chem.}, keywords = {barley, lunasin}, month = {October}, number = {21}, pages = {5903--5908}, posted-at = {2008-04-10 16:28:00}, priority = {2}, title = {Barley Lunasin Suppresses ras-Induced Colony Formation and Inhibits Core Histone Acetylation in Mammalian Cells}, url = {http://dx.doi.org/10.1021/jf0256945}, volume = {50}, year = {2002} }

TY - JOUR ID - citeulike:2649745 L3 - citeulike-article-id:2649745 N2 - Abstract: Lunasin is a novel peptide originally identified in soybean that suppresses chemical carcinogen-induced transformation in mammalian cells and skin carcinogenesis in mice. Since the lunasin gene was cloned from soybean and the chemically synthesized form of the lunasin peptide has been used in experiments conducted so far, the isolation of lunasin from other natural sources and testing of its biological properties have not been carried out. We report here the isolation, purification, and biological assay of lunasin from barley, a newly found rich source of the peptide. The identity of lunasin was established by Western blot analysis and mass spectrometric peptide mapping of the in-gel tryptic digest of the putative protein band. Lunasin was partially purified with anion exchange and immunoaffinity chromatography. The crude and partially purified lunasin from barley suppressed colony formation in stably ras-transfected mouse fibroblast cells induced with IPTG. These fractions also inhibited histone acetylation in mouse fibroblast NIH 3T3 and human breast MCF-7 cells in the presence of the histone deacetylase inhibitor sodium butyrate. Keywords: Barley lunasin; chemopreventive peptide; isolation; purification and bioassay; mass spectrometric peptide mapping; suppression of colony formation; ras oncogene; inhibition of histone acetylation IS - 21 JF - J. Agric. Food Chem. AD - School of Bioresources, University of Andong, Kyungpook, Korea, BioRad Laboratories, Inc., Hercules, California, and Department of Nutritional Sciences & Toxicology, University of California, Berkeley, California 94720 EP - 5908 TI - Barley Lunasin Suppresses ras-Induced Colony Formation and Inhibits Core Histone Acetylation in Mammalian Cells VL - 50 SP - 5903 KW - barley KW - lunasin AU - Jeong, HJ AU - Lam, Y AU - de Lumen, BO PY - 2002/10/09/ UR - http://dx.doi.org/10.1021/jf0256945 ER -