Expression and localization of human lysozyme in the endosperm of transgenic rice. Export

@article{citeulike:3180035, abstract = {In order to understand the characteristics of recombinant protein expression and sublocalization in rice ( Oryza sativa L.) endosperm, we examined the expression level of human lysozyme protein and its subcellular location in transgenic rice seeds driven by rice glutelin and globulin promoters and signal peptides. A time course of human lysozyme expression during endosperm development was analyzed. The results showed that the expression profile of recombinant protein accumulation in endosperm paralleled that of the two storage proteins. Immunofluorescence microscopy revealed that human lysozyme and storage proteins co-localized to type-II protein bodies. Both promoter-signal peptide parings targeted recombinant protein to the protein bodies. In addition, a transgenic line with a higher lysozyme expression level exhibited morphologically different protein bodies with an unbalanced composition of lysozyme and native storage proteins. The high-level expression of recombinant protein distorted the trafficking and sorting of native storage proteins in rice endosperm and affected the expression of native storage protein.}, address = {Ventria Bioscience, 4110 North Freeway, Sacramento, CA 95834, USA. dyang@ventriabio.com}, author = {Yang, D. and Guo, F. and Liu, B. and Huang, N. and Watkins, S. C.}, citeulike-article-id = {3180035}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00425-002-0919-x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12569401}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12569401}, doi = {10.1007/s00425-002-0919-x}, issn = {0032-0935}, journal = {Planta}, keywords = {rice, ventria}, month = {February}, number = {4}, pages = {597--603}, posted-at = {2008-09-01 22:31:45}, priority = {0}, title = {Expression and localization of human lysozyme in the endosperm of transgenic rice.}, url = {http://dx.doi.org/10.1007/s00425-002-0919-x}, volume = {216}, year = {2003} }

TY - JOUR ID - citeulike:3180035 L3 - citeulike-article-id:3180035 N2 - In order to understand the characteristics of recombinant protein expression and sublocalization in rice ( Oryza sativa L.) endosperm, we examined the expression level of human lysozyme protein and its subcellular location in transgenic rice seeds driven by rice glutelin and globulin promoters and signal peptides. A time course of human lysozyme expression during endosperm development was analyzed. The results showed that the expression profile of recombinant protein accumulation in endosperm paralleled that of the two storage proteins. Immunofluorescence microscopy revealed that human lysozyme and storage proteins co-localized to type-II protein bodies. Both promoter-signal peptide parings targeted recombinant protein to the protein bodies. In addition, a transgenic line with a higher lysozyme expression level exhibited morphologically different protein bodies with an unbalanced composition of lysozyme and native storage proteins. The high-level expression of recombinant protein distorted the trafficking and sorting of native storage proteins in rice endosperm and affected the expression of native storage protein. IS - 4 JF - Planta SN - 0032-0935 AD - Ventria Bioscience, 4110 North Freeway, Sacramento, CA 95834, USA. dyang@ventriabio.com EP - 603 TI - Expression and localization of human lysozyme in the endosperm of transgenic rice. VL - 216 SP - 597 KW - rice KW - ventria AU - Yang, D AU - Guo, F AU - Liu, B AU - Huang, N AU - Watkins, SC PY - 2003/02// UR - http://dx.doi.org/10.1007/s00425-002-0919-x ER -