Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation. Export

@article{citeulike:5518049, abstract = {We developed an asexual reproductive plant, Kalanchoe pinnata, as a new bioreactor for plant-based molecular farming using a newly developed transformation method. Leaf crenate margins were pin-pricked to infect the plant with the Agrobacterium strain LBA4404 and vacuum infiltration was also applied to introduce the target gene into the plants. Subsequently, the young mother leaf produced new clones at the leaf crenate margins without the need for time- and labor-consuming tissue culture procedures. The average transformation rates were approximately 77 and 84\% for pin-prickling and vacuum-infiltration methods, respectively. To functionally characterize an introduced target protein, a nucleic acid hydrolyzing recombinant 3D8 scFv was selected and the plant based 3D8 scFv proteins were purified and analyzed. Based on abzyme analysis, the purified protein expressed with this system had catalytic activity and exhibited all of properties of the protein produced in an E. coli system. This result suggested that vegetatively reproductive K. pinnata can be a novel and potent bioreactor for bio-pharmaceutical proteins.}, author = {Jung, Yuchul and Rhee, Yong and Auh, Chung-Kyoon K. and Shim, Hyekyung and Choi, Jung-Jin J. and Kwon, Suk-Tae T. and Yang, Joo-Sung S. and Kim, Donggiun and Kwon, Myung-Hee H. and Kim, Yong-Sung S. and Lee, Sukchan}, citeulike-article-id = {5518049}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00299-009-0758-3}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19688214}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19688214}, citeulike-linkout-3 = {http://www.springerlink.com/content/308706641253l235}, doi = {10.1007/s00299-009-0758-3}, issn = {1432-203X}, journal = {Plant cell reports}, keywords = {antibody, pmp, transgenic}, month = {October}, number = {10}, pages = {1593--1602}, posted-at = {2009-09-28 17:30:38}, priority = {0}, title = {Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation.}, url = {http://dx.doi.org/10.1007/s00299-009-0758-3}, volume = {28}, year = {2009} }

TY - JOUR ID - citeulike:5518049 L3 - citeulike-article-id:5518049 N2 - We developed an asexual reproductive plant, Kalanchoe pinnata, as a new bioreactor for plant-based molecular farming using a newly developed transformation method. Leaf crenate margins were pin-pricked to infect the plant with the Agrobacterium strain LBA4404 and vacuum infiltration was also applied to introduce the target gene into the plants. Subsequently, the young mother leaf produced new clones at the leaf crenate margins without the need for time- and labor-consuming tissue culture procedures. The average transformation rates were approximately 77 and 84% for pin-prickling and vacuum-infiltration methods, respectively. To functionally characterize an introduced target protein, a nucleic acid hydrolyzing recombinant 3D8 scFv was selected and the plant based 3D8 scFv proteins were purified and analyzed. Based on abzyme analysis, the purified protein expressed with this system had catalytic activity and exhibited all of properties of the protein produced in an E. coli system. This result suggested that vegetatively reproductive K. pinnata can be a novel and potent bioreactor for bio-pharmaceutical proteins. IS - 10 JF - Plant cell reports SN - 1432-203X EP - 1602 TI - Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation. VL - 28 SP - 1593 KW - antibody KW - pmp KW - transgenic AU - Jung, Yuchul AU - Rhee, Yong AU - Auh, Chung-Kyoon AU - Shim, Hyekyung AU - Choi, Jung-Jin AU - Kwon, Suk-Tae AU - Yang, Joo-Sung AU - Kim, Donggiun AU - Kwon, Myung-Hee AU - Kim, Yong-Sung AU - Lee, Sukchan PY - 2009/10// UR - http://dx.doi.org/10.1007/s00299-009-0758-3 ER -