Refinement of Protein Structures into Low-Resolution Density Maps Using Rosetta Export

@article{citeulike:5139417, abstract = {We describe a method based on Rosetta structure refinement for generating high-resolution, all-atom protein models from electron cryomicroscopy density maps. A local measure of the fit of a model to the density is used to directly guide structure refinement and to identify regions incompatible with the density that are then targeted for extensive rebuilding. Over a range of test cases using both simulated and experimentally generated data, the method consistently increases the accuracy of starting models generated either by comparative modeling or by hand-tracing the density. The method can achieve near-atomic resolution starting from density maps at 4–6 \r{A} resolution.}, author = {Dimaio, Frank and Tyka, Michael D. and Baker, Matthew L. and Chiu, Wah and Baker, David}, citeulike-article-id = {5139417}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jmb.2009.07.008}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0022283609008341}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19596339}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19596339}, day = {08}, doi = {10.1016/j.jmb.2009.07.008}, issn = {00222836}, journal = {Journal of Molecular Biology}, keywords = {cryo\_em, protein\_protein}, month = {July}, posted-at = {2009-07-26 09:05:37}, priority = {2}, title = {Refinement of Protein Structures into Low-Resolution Density Maps Using Rosetta}, url = {http://dx.doi.org/10.1016/j.jmb.2009.07.008}, year = {2009} }

TY - JOUR ID - citeulike:5139417 L3 - citeulike-article-id:5139417 N2 - We describe a method based on Rosetta structure refinement for generating high-resolution, all-atom protein models from electron cryomicroscopy density maps. A local measure of the fit of a model to the density is used to directly guide structure refinement and to identify regions incompatible with the density that are then targeted for extensive rebuilding. Over a range of test cases using both simulated and experimentally generated data, the method consistently increases the accuracy of starting models generated either by comparative modeling or by hand-tracing the density. The method can achieve near-atomic resolution starting from density maps at 4–6 Å resolution. JF - Journal of Molecular Biology SN - 00222836 TI - Refinement of Protein Structures into Low-Resolution Density Maps Using Rosetta KW - cryo_em KW - protein_protein AU - Dimaio, Frank AU - Tyka, Michael AU - Baker, Matthew AU - Chiu, Wah AU - Baker, David PY - 2009/07/08/ UR - http://dx.doi.org/10.1016/j.jmb.2009.07.008 ER -