Methicillin-Resistant Staphylococcus aureus in Spain: Molecular Epidemiology and Utility of Different Typing Methods Export

@article{citeulike:4680961, abstract = {In a point-prevalence study performed in 145 Spanish hospitals in 2006, we collected 463 isolates of Staphylococcus aureus in a single day. Of these, 135 (29.2\%) were methicillin (meticillin)-resistant S. aureus (MRSA) isolates. Susceptibility testing was performed by a microdilution method, and mecA was detected by PCR. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE) after SmaI digestion, staphylococcal chromosomal cassette mec (SCCmec) typing, agr typing, spa typing with BURP (based-upon-repeat-pattern) analysis, and multilocus sequence typing (MLST). The 135 MRSA isolates showed resistance to ciprofloxacin (93.3\%), tobramycin (72.6\%), gentamicin (20.0\%), erythromycin (66.7\%), and clindamycin (39.3\%). Among the isolates resistant to erythromycin, 27.4\% showed the M phenotype. All of the isolates were susceptible to glycopeptides. Twelve resistance patterns were found, of which four accounted for 65\% of the isolates. PFGE revealed 36 different patterns, with 13 major clones (including 2 predominant clones with various antibiotypes that accounted for 52.5\% of the MRSA isolates) and 23 sporadic profiles. Two genotypes were observed for the first time in Spain. SCCmec type IV accounted for 6.7\% of the isolates (70.1\% were type IVa, 23.9\% were type IVc, 0.9\% were type IVd, and 5.1\% were type IVh), and SCCmec type I and SCCmec type II accounted for 7.4\% and 5.2\% of the isolates, respectively. One isolate was nontypeable. Only one of the isolates produced the Panton-Valentine leukocidin. The isolates presented agr type 2 (82.2\%), type 1 (14.8\%), and type 3 (3.0\%). spa typing revealed 32 different types, the predominant ones being t067 (48.9\%) and t002 (14.8\%), as well as clonal complex 067 (78\%) by BURP analysis. The MRSA clone of sequence type 125 and SCCmec type IV was the most prevalent throughout Spain. In our experience, PFGE, spa typing, SCCmec typing, and MLST presented good correlations for the majority of the MRSA strains; we suggest the use of spa typing and PFGE typing for epidemiological surveillance, since this combination is useful for both long-term and short-term studies. 10.1128/JCM.01579-08}, author = {Vindel, Ana and Cuevas, Oscar and Cercenado, Emilia and Marcos, Carmen and Bautista, Veronica and Castellares, Carol and Trincado, Pilar and Boquete, Teresa and Perez-Vazquez, Maria and Marin, Mercedes and Bouza, Emilio and And}, citeulike-article-id = {4680961}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JCM.01579-08}, citeulike-linkout-1 = {http://jcm.asm.org/cgi/content/abstract/47/6/1620?etoc}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19339473}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19339473}, day = {1}, doi = {10.1128/JCM.01579-08}, journal = {J. Clin. Microbiol.}, keywords = {mrsa, typing}, month = {June}, number = {6}, pages = {1620--1627}, posted-at = {2009-05-30 06:54:42}, priority = {1}, title = {Methicillin-Resistant Staphylococcus aureus in Spain: Molecular Epidemiology and Utility of Different Typing Methods}, url = {http://dx.doi.org/10.1128/JCM.01579-08}, volume = {47}, year = {2009} }

TY - JOUR ID - citeulike:4680961 L3 - citeulike-article-id:4680961 N2 - In a point-prevalence study performed in 145 Spanish hospitals in 2006, we collected 463 isolates of Staphylococcus aureus in a single day. Of these, 135 (29.2%) were methicillin (meticillin)-resistant S. aureus (MRSA) isolates. Susceptibility testing was performed by a microdilution method, and mecA was detected by PCR. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE) after SmaI digestion, staphylococcal chromosomal cassette mec (SCCmec) typing, agr typing, spa typing with BURP (based-upon-repeat-pattern) analysis, and multilocus sequence typing (MLST). The 135 MRSA isolates showed resistance to ciprofloxacin (93.3%), tobramycin (72.6%), gentamicin (20.0%), erythromycin (66.7%), and clindamycin (39.3%). Among the isolates resistant to erythromycin, 27.4% showed the M phenotype. All of the isolates were susceptible to glycopeptides. Twelve resistance patterns were found, of which four accounted for 65% of the isolates. PFGE revealed 36 different patterns, with 13 major clones (including 2 predominant clones with various antibiotypes that accounted for 52.5% of the MRSA isolates) and 23 sporadic profiles. Two genotypes were observed for the first time in Spain. SCCmec type IV accounted for 6.7% of the isolates (70.1% were type IVa, 23.9% were type IVc, 0.9% were type IVd, and 5.1% were type IVh), and SCCmec type I and SCCmec type II accounted for 7.4% and 5.2% of the isolates, respectively. One isolate was nontypeable. Only one of the isolates produced the Panton-Valentine leukocidin. The isolates presented agr type 2 (82.2%), type 1 (14.8%), and type 3 (3.0%). spa typing revealed 32 different types, the predominant ones being t067 (48.9%) and t002 (14.8%), as well as clonal complex 067 (78%) by BURP analysis. The MRSA clone of sequence type 125 and SCCmec type IV was the most prevalent throughout Spain. In our experience, PFGE, spa typing, SCCmec typing, and MLST presented good correlations for the majority of the MRSA strains; we suggest the use of spa typing and PFGE typing for epidemiological surveillance, since this combination is useful for both long-term and short-term studies. 10.1128/JCM.01579-08 IS - 6 JF - J. Clin. Microbiol. EP - 1627 TI - Methicillin-Resistant Staphylococcus aureus in Spain: Molecular Epidemiology and Utility of Different Typing Methods VL - 47 SP - 1620 KW - mrsa KW - typing AU - Vindel, Ana AU - Cuevas, Oscar AU - Cercenado, Emilia AU - Marcos, Carmen AU - Bautista, Veronica AU - Castellares, Carol AU - Trincado, Pilar AU - Boquete, Teresa AU - Perez-Vazquez, Maria AU - Marin, Mercedes AU - Bouza, Emilio AU - And PY - 2009/06/01/ UR - http://dx.doi.org/10.1128/JCM.01579-08 ER -