Capsular Localization of the Cryptococcus neoformans Polysaccharide Component Galactoxylomannan. Export

@article{citeulike:3457774, abstract = {Cryptococcus neoformans capsular polysaccharide is composed of at least two components, glucuronoxylomannan (GXM) and galactoxylomannans (GalXM). Although GXM has been extensively studied, little is known about the location of GalXM in the C. neoformans capsule, in part because there are no serological reagents specific to this antigen. To circumvent the poor immunogenicity of GalXM, this antigen was conjugated to Protective Antigen (PA) from Bacillus anthracis as a protein carrier. The resulting conjugate elicited antibodies that reacted with GalXM in mice and yielded an immune serum that proved useful for studying GalXM in the polysaccharide capsule. In acapsular cells, immune serum localized GalXM to the cell wall. In capsulated cells, immune serum localized GalXM to discrete pockets near the capsule edge. GalXM was abundant on the nascent capsules of budding daughter cells. The constituent sugars of GalXM were found in vesicle fractions consistent with vesicular transport for this polysaccharide. In addition, we generated a single chain fraction variable (scFv) fragment antibody with a specificity to oxidized carbohydrates that also produced punctate immunofluorescence on encapsulated cells that partially co-localized with GalXM. The results are interpreted to mean that GalXM is a transient component of the polysaccharide capsule of mature cells during the process of secretion. Hence, the function of GalXM appears to be more consistent with that of an exopolysaccharide, than a structural component of the cryptococcal capsule.}, author = {De Jesus, Magdia and Nicola, Andr\'{e} Moraes M. and Rodrigues, Marcio L L. and Janbon, Guilhem and Casadevall, Arturo}, citeulike-article-id = {3457774}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/EC.00331-08}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18952901}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18952901}, day = {24}, doi = {10.1128/EC.00331-08}, issn = {1535-9786}, journal = {Eukaryotic cell}, keywords = {cryptococcus, ecm, galactoxylomannan}, month = {October}, posted-at = {2008-10-28 15:57:19}, priority = {2}, title = {Capsular Localization of the Cryptococcus neoformans Polysaccharide Component Galactoxylomannan.}, url = {http://dx.doi.org/10.1128/EC.00331-08}, year = {2008} }

TY - JOUR ID - citeulike:3457774 L3 - citeulike-article-id:3457774 N2 - Cryptococcus neoformans capsular polysaccharide is composed of at least two components, glucuronoxylomannan (GXM) and galactoxylomannans (GalXM). Although GXM has been extensively studied, little is known about the location of GalXM in the C. neoformans capsule, in part because there are no serological reagents specific to this antigen. To circumvent the poor immunogenicity of GalXM, this antigen was conjugated to Protective Antigen (PA) from Bacillus anthracis as a protein carrier. The resulting conjugate elicited antibodies that reacted with GalXM in mice and yielded an immune serum that proved useful for studying GalXM in the polysaccharide capsule. In acapsular cells, immune serum localized GalXM to the cell wall. In capsulated cells, immune serum localized GalXM to discrete pockets near the capsule edge. GalXM was abundant on the nascent capsules of budding daughter cells. The constituent sugars of GalXM were found in vesicle fractions consistent with vesicular transport for this polysaccharide. In addition, we generated a single chain fraction variable (scFv) fragment antibody with a specificity to oxidized carbohydrates that also produced punctate immunofluorescence on encapsulated cells that partially co-localized with GalXM. The results are interpreted to mean that GalXM is a transient component of the polysaccharide capsule of mature cells during the process of secretion. Hence, the function of GalXM appears to be more consistent with that of an exopolysaccharide, than a structural component of the cryptococcal capsule. JF - Eukaryotic cell SN - 1535-9786 TI - Capsular Localization of the Cryptococcus neoformans Polysaccharide Component Galactoxylomannan. KW - cryptococcus KW - ecm KW - galactoxylomannan AU - De Jesus, Magdia AU - Nicola, André Moraes AU - Rodrigues, Marcio L AU - Janbon, Guilhem AU - Casadevall, Arturo PY - 2008/10/24/ UR - http://dx.doi.org/10.1128/EC.00331-08 ER -