A MAP kinase cascade required for regulation of development and secondary metabolism in Neurospora crassa. Export

@article{citeulike:3475282, abstract = {Mitogen-activated protein (MAP) kinase signaling cascades are composed of MAP kinase kinase kinases (MAPKKKs), MAP kinase kinases (MAPKKs) and MAP kinases (MAPKs). In this study, we characterize components of a MAPK cascade in Neurospora crassa (mik-1, MAPKKK; mek-1, MAPKK; mak-1, MAPK), homologous to that controlling cell wall integrity in Saccharomyces cerevisiae. Growth of basal hyphae is significantly reduced in mik-1, mek-1, and mak-1 deletion mutants on solid medium. All three mutants formed short aerial hyphae and formation of asexual macroconidia was reduced in Deltamik-1 mutants and almost abolished in Deltamek-1 and Deltamak-1 strains. In contrast, the normally rare asexual spores, arthroconidia, were abundant in cultures of the three mutants. Deltamik-1, Deltamek-1, and Deltamak-1 mutants were unable to form protoperithecia or perithecia when used as females in a sexual cross. The MAK-1 MAPK was not phosphorylated in Deltamik-1 and Deltamek-1 mutants, consistent with involvement of MIK-1, MEK-1 and MAK-1 in the same signaling cascade. Interestingly, we observed increased levels of mRNA and protein for tyrosinase in the mutants under nitrogen starvation, a condition favoring sexual differentiation. Tyrosinase is an enzyme that catalyzes production of the secondary metabolite L-DOPA melanin. These results implicate the MAK-1 pathway in regulation of development and secondary metabolism in filamentous fungi.}, author = {Park, Gyungsoon and Pan, Songqin and Borkovich, Katherine A A.}, citeulike-article-id = {3475282}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/EC.00466-07}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18849472}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18849472}, day = {10}, doi = {10.1128/EC.00466-07}, issn = {1535-9786}, journal = {Eukaryotic cell}, keywords = {map-kinase, neurospora-crassa}, month = {October}, posted-at = {2008-11-03 15:14:30}, priority = {2}, title = {A MAP kinase cascade required for regulation of development and secondary metabolism in Neurospora crassa.}, url = {http://dx.doi.org/10.1128/EC.00466-07}, year = {2008} }

TY - JOUR ID - citeulike:3475282 L3 - citeulike-article-id:3475282 N2 - Mitogen-activated protein (MAP) kinase signaling cascades are composed of MAP kinase kinase kinases (MAPKKKs), MAP kinase kinases (MAPKKs) and MAP kinases (MAPKs). In this study, we characterize components of a MAPK cascade in Neurospora crassa (mik-1, MAPKKK; mek-1, MAPKK; mak-1, MAPK), homologous to that controlling cell wall integrity in Saccharomyces cerevisiae. Growth of basal hyphae is significantly reduced in mik-1, mek-1, and mak-1 deletion mutants on solid medium. All three mutants formed short aerial hyphae and formation of asexual macroconidia was reduced in Deltamik-1 mutants and almost abolished in Deltamek-1 and Deltamak-1 strains. In contrast, the normally rare asexual spores, arthroconidia, were abundant in cultures of the three mutants. Deltamik-1, Deltamek-1, and Deltamak-1 mutants were unable to form protoperithecia or perithecia when used as females in a sexual cross. The MAK-1 MAPK was not phosphorylated in Deltamik-1 and Deltamek-1 mutants, consistent with involvement of MIK-1, MEK-1 and MAK-1 in the same signaling cascade. Interestingly, we observed increased levels of mRNA and protein for tyrosinase in the mutants under nitrogen starvation, a condition favoring sexual differentiation. Tyrosinase is an enzyme that catalyzes production of the secondary metabolite L-DOPA melanin. These results implicate the MAK-1 pathway in regulation of development and secondary metabolism in filamentous fungi. JF - Eukaryotic cell SN - 1535-9786 TI - A MAP kinase cascade required for regulation of development and secondary metabolism in Neurospora crassa. KW - map-kinase KW - neurospora-crassa AU - Park, Gyungsoon AU - Pan, Songqin AU - Borkovich, Katherine A PY - 2008/10/10/ UR - http://dx.doi.org/10.1128/EC.00466-07 ER -