A Putative Polyketide Synthase/Peptide Synthetase from Magnaporthe grisea Signals Pathogen Attack to Resistant Rice Export

@article{citeulike:4061377, abstract = {Isolates of the rice blast fungus Magnaporthe grisea that carry the gene encoding Avirulence Conferring Enzyme1 (ACE1) are specifically recognized by rice (Oryza sativa) cultivars carrying the resistance gene Pi33. This recognition enables resistant plants to activate a defense response. ACE1 was isolated by map-based cloning and encodes a putative hybrid between a polyketide synthase and a nonribosomal peptide synthetase, enzymes involved in microbial secondary metabolism. ACE1 is expressed exclusively during fungal penetration of host leaves, the time point at which plant defense reactions are triggered. Ace1 appears to be localized in the cytoplasm of the appressorium. Mutation of the putative catalytic site of the {beta}-ketoacyl synthase domain of Ace1 abolishes recognition of the fungus by resistant rice. This suggests that Ace1 biosynthetic activity is required for avirulence. Our results are consistent with the hypothesis that the fungal signal recognized by resistant rice plants is the secondary metabolite whose synthesis depends on Ace1. 10.1105/tpc.104.022715}, author = {Bohnert, Heidi U. and Fudal, Isabelle and Dioh, Waly and Tharreau, Didier and Notteghem, Jean-Loup and Lebrun, Marc-Henri}, citeulike-article-id = {4061377}, citeulike-linkout-0 = {http://dx.doi.org/10.1105/tpc.104.022715}, citeulike-linkout-1 = {http://www.plantcell.org/cgi/content/abstract/16/9/2499}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15319478}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15319478}, day = {1}, doi = {10.1105/tpc.104.022715}, journal = {Plant Cell}, keywords = {magnaporthe, nps, pks}, month = {September}, number = {9}, pages = {2499--2513}, posted-at = {2009-02-16 21:16:21}, priority = {2}, title = {A Putative Polyketide Synthase/Peptide Synthetase from Magnaporthe grisea Signals Pathogen Attack to Resistant Rice}, url = {http://dx.doi.org/10.1105/tpc.104.022715}, volume = {16}, year = {2004} }

TY - JOUR ID - citeulike:4061377 L3 - citeulike-article-id:4061377 N2 - Isolates of the rice blast fungus Magnaporthe grisea that carry the gene encoding Avirulence Conferring Enzyme1 (ACE1) are specifically recognized by rice (Oryza sativa) cultivars carrying the resistance gene Pi33. This recognition enables resistant plants to activate a defense response. ACE1 was isolated by map-based cloning and encodes a putative hybrid between a polyketide synthase and a nonribosomal peptide synthetase, enzymes involved in microbial secondary metabolism. ACE1 is expressed exclusively during fungal penetration of host leaves, the time point at which plant defense reactions are triggered. Ace1 appears to be localized in the cytoplasm of the appressorium. Mutation of the putative catalytic site of the {beta}-ketoacyl synthase domain of Ace1 abolishes recognition of the fungus by resistant rice. This suggests that Ace1 biosynthetic activity is required for avirulence. Our results are consistent with the hypothesis that the fungal signal recognized by resistant rice plants is the secondary metabolite whose synthesis depends on Ace1. 10.1105/tpc.104.022715 IS - 9 JF - Plant Cell EP - 2513 TI - A Putative Polyketide Synthase/Peptide Synthetase from Magnaporthe grisea Signals Pathogen Attack to Resistant Rice VL - 16 SP - 2499 KW - magnaporthe KW - nps KW - pks AU - Bohnert, Heidi AU - Fudal, Isabelle AU - Dioh, Waly AU - Tharreau, Didier AU - Notteghem, Jean-Loup AU - Lebrun, Marc-Henri PY - 2004/09/01/ UR - http://dx.doi.org/10.1105/tpc.104.022715 ER -