The ER Chaperone LHS1 Is Involved in Asexual Development and Rice Infection by the Blast Fungus Magnaporthe oryzae Export

@article{citeulike:4292723, abstract = {In planta secretion of fungal pathogen proteins, including effectors destined for the plant cell cytoplasm, is critical for disease progression. However, little is known about the endoplasmic reticulum (ER) secretion mechanisms used by these pathogens. To determine if normal ER function is crucial for fungal pathogenicity, Magnaporthe oryzae genes encoding proteins homologous to yeast Lhs1p and Kar2p, members of the heat shock protein 70 family in Saccharomyces cerevisiae, were cloned and characterized. Like their yeast counterparts, both LHS1 and KAR2 proteins localized in the ER and functioned in an unfolded protein response (UPR) similar to the yeast UPR. Mutants produced by disruption of LHS1 were viable but showed a defect in the translocation of proteins across the ER membrane and reduced activities of extracellular enzymes. The{Delta} lhs1 mutant was severely impaired not only in conidiation, but also in both penetration and biotrophic invasion in susceptible rice (Oryza sativa) plants. This mutant also had defects in the induction of the Pi-ta resistance gene-mediated hypersensitive response and in the accumulation of fluorescently-labeled secreted effector proteins in biotrophic interfacial complexes. Our results suggest that proper processing of secreted proteins, including effectors, by chaperones in the ER is requisite for successful disease development and for determining host-pathogen compatibility via the gene-for-gene interaction. 10.1105/tpc.107.055988}, author = {Yi, Mihwa and Chi, Myoung-Hwan and Khang, Chang H. and Park, Sook-Young and Kang, Seogchan and Valent, Barbara and Lee, Yong-Hwan}, citeulike-article-id = {4292723}, citeulike-linkout-0 = {http://dx.doi.org/10.1105/tpc.107.055988}, citeulike-linkout-1 = {http://www.plantcell.org/cgi/content/abstract/21/2/681?rss=1}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19252083}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19252083}, day = {1}, doi = {10.1105/tpc.107.055988}, journal = {Plant Cell}, keywords = {magnaporthe, valent}, month = {February}, number = {2}, pages = {681--695}, posted-at = {2009-04-08 23:28:54}, priority = {2}, title = {The ER Chaperone LHS1 Is Involved in Asexual Development and Rice Infection by the Blast Fungus Magnaporthe oryzae}, url = {http://dx.doi.org/10.1105/tpc.107.055988}, volume = {21}, year = {2009} }

TY - JOUR ID - citeulike:4292723 L3 - citeulike-article-id:4292723 N2 - In planta secretion of fungal pathogen proteins, including effectors destined for the plant cell cytoplasm, is critical for disease progression. However, little is known about the endoplasmic reticulum (ER) secretion mechanisms used by these pathogens. To determine if normal ER function is crucial for fungal pathogenicity, Magnaporthe oryzae genes encoding proteins homologous to yeast Lhs1p and Kar2p, members of the heat shock protein 70 family in Saccharomyces cerevisiae, were cloned and characterized. Like their yeast counterparts, both LHS1 and KAR2 proteins localized in the ER and functioned in an unfolded protein response (UPR) similar to the yeast UPR. Mutants produced by disruption of LHS1 were viable but showed a defect in the translocation of proteins across the ER membrane and reduced activities of extracellular enzymes. The{Delta} lhs1 mutant was severely impaired not only in conidiation, but also in both penetration and biotrophic invasion in susceptible rice (Oryza sativa) plants. This mutant also had defects in the induction of the Pi-ta resistance gene-mediated hypersensitive response and in the accumulation of fluorescently-labeled secreted effector proteins in biotrophic interfacial complexes. Our results suggest that proper processing of secreted proteins, including effectors, by chaperones in the ER is requisite for successful disease development and for determining host-pathogen compatibility via the gene-for-gene interaction. 10.1105/tpc.107.055988 IS - 2 JF - Plant Cell EP - 695 TI - The ER Chaperone LHS1 Is Involved in Asexual Development and Rice Infection by the Blast Fungus Magnaporthe oryzae VL - 21 SP - 681 KW - magnaporthe KW - valent AU - Yi, Mihwa AU - Chi, Myoung-Hwan AU - Khang, Chang AU - Park, Sook-Young AU - Kang, Seogchan AU - Valent, Barbara AU - Lee, Yong-Hwan PY - 2009/02/01/ UR - http://dx.doi.org/10.1105/tpc.107.055988 ER -