Galpha and Gbeta proteins regulate the cAMP pathway that is required for development and pathogenicity of the phytopathogen Mycosphaerella graminicola. Export

@article{citeulike:4480520, abstract = {We identified and functionally characterized genes encoding three Galpha proteins and one Gbeta protein in the dimorphic fungal wheat pathogen Mycosphaerella graminicola, which we designated MgGpa1, MgGpa2, MgGpa3 and MgGpb1, respectively. Sequence comparisons and phylogenetic analyses showed that MgGPA1 and MgGPA3 are most related to the mammalian Galphai and Galphas families, respectively, whereas MgGPA2 is not related to either of these two families. On PDA and in yeast glucose broth (YGB), MgGpa1 mutants produced significantly longer spores than the WT, that developed into unique fluffy mycelia in the latter medium, indicating that this gene negatively controls filamentation. MgGpa3 mutants showed more pronounced yeast-like growth accompanied with hampered filamentation and secreted a dark-brown pigment into YGB. Germ tubes emerging from spores of MgGpb1 mutants were wavy on water agar (WA) and showed a nested-type of growth on PDA that was due to hampered filamentation, numerous cell fusions and increased anastomosis. Intracellular cAMP levels of MgGpb1 and MgGpa3 mutants were decreased indicating that both genes positively regulate the cAMP pathway, which was confirmed as the WT phenotype was restored by adding cAMP to these mutant cultures. The cAMP levels in MgGpa1 mutants and the WT were not significantly different, suggesting that this gene might be dispensable for cAMP regulation. In planta assays showed that mutants of MgGpa1, MgGpa3 and MgGpb1 are strongly reduced in pathogenicity. We conclude that the heterotrimeric G proteins MgGpa3 and MgGpb1 regulate the cAMP pathway that is required for development and pathogenicity in M. graminicola.}, author = {Mehrabi, Rahim and M'barek, Sarrah Ben B. and van der Lee, Theo A J A. and Waalwijk, Cees and de Wit, Pierre J G M J. and Kema, Gerrit H J H.}, citeulike-article-id = {4480520}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/EC.00258-08}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19411619}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19411619}, day = {1}, doi = {10.1128/EC.00258-08}, issn = {1535-9786}, journal = {Eukaryotic cell}, keywords = {camp, gproteins, mgraminicola}, month = {May}, posted-at = {2009-05-06 19:18:47}, priority = {2}, title = {G{alpha} and G{beta} proteins regulate the cAMP pathway that is required for development and pathogenicity of the phytopathogen Mycosphaerella graminicola.}, url = {http://dx.doi.org/10.1128/EC.00258-08}, year = {2009} }

TY - JOUR ID - citeulike:4480520 L3 - citeulike-article-id:4480520 N2 - We identified and functionally characterized genes encoding three Galpha proteins and one Gbeta protein in the dimorphic fungal wheat pathogen Mycosphaerella graminicola, which we designated MgGpa1, MgGpa2, MgGpa3 and MgGpb1, respectively. Sequence comparisons and phylogenetic analyses showed that MgGPA1 and MgGPA3 are most related to the mammalian Galphai and Galphas families, respectively, whereas MgGPA2 is not related to either of these two families. On PDA and in yeast glucose broth (YGB), MgGpa1 mutants produced significantly longer spores than the WT, that developed into unique fluffy mycelia in the latter medium, indicating that this gene negatively controls filamentation. MgGpa3 mutants showed more pronounced yeast-like growth accompanied with hampered filamentation and secreted a dark-brown pigment into YGB. Germ tubes emerging from spores of MgGpb1 mutants were wavy on water agar (WA) and showed a nested-type of growth on PDA that was due to hampered filamentation, numerous cell fusions and increased anastomosis. Intracellular cAMP levels of MgGpb1 and MgGpa3 mutants were decreased indicating that both genes positively regulate the cAMP pathway, which was confirmed as the WT phenotype was restored by adding cAMP to these mutant cultures. The cAMP levels in MgGpa1 mutants and the WT were not significantly different, suggesting that this gene might be dispensable for cAMP regulation. In planta assays showed that mutants of MgGpa1, MgGpa3 and MgGpb1 are strongly reduced in pathogenicity. We conclude that the heterotrimeric G proteins MgGpa3 and MgGpb1 regulate the cAMP pathway that is required for development and pathogenicity in M. graminicola. JF - Eukaryotic cell SN - 1535-9786 TI - G{alpha} and G{beta} proteins regulate the cAMP pathway that is required for development and pathogenicity of the phytopathogen Mycosphaerella graminicola. KW - camp KW - gproteins KW - mgraminicola AU - Mehrabi, Rahim AU - M'barek, Sarrah Ben AU - van der Lee, Theo A J AU - Waalwijk, Cees AU - de Wit, Pierre J G M AU - Kema, Gerrit H J PY - 2009/05/01/ UR - http://dx.doi.org/10.1128/EC.00258-08 ER -