The interplay between iron and zinc metabolism in Aspergillus fumigatus. Export

@article{citeulike:4836641, abstract = {Zinc plays a critical role in a diverse array of biochemical processes. However, excess of zinc is deleterious to cells. Therefore, cells require finely tuned homeostatic mechanisms to balance uptake and storage of zinc. Here we show that iron starvation affects zinc metabolism by downregulating expression of the plasma membrane zinc importer encoding zrfB and upregulating the putative vacuolar zinc transporter-encoding zrcA in Aspergillus fumigatus. Nevertheless, the zinc content of iron-starved mycelia exceeded that of iron replete mycelia, possibly due to unspecific metal uptake induced by iron starvation. In agreement with increased zinc excess and zinc toxicity during iron starvation, deficiency in siderophore-mediated high-affinity iron uptake caused hypersensitivity to zinc. Moreover, an increase of zinc uptake by conditional overexpression of zrfB was more toxic under iron depleted compared to iron replete conditions. This deregulated zinc uptake under iron starvation caused a decrease in heme production and an increase in protoporphyrin IX accumulation. Furthermore, zinc excess impaired production of the extracellular siderophore triacetylfusarinine C but not the intracellular siderophore ferricrocin. Taken together, these data demonstrate a fine tuned coordination of zinc and iron metabolism in A. fumigatus.}, author = {Yasmin, Sabiha and Abt, Beate and Schrettl, Markus and Moussa, Tarek A. and Werner, Ernst R. and Haas, Hubertus}, citeulike-article-id = {4836641}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.fgb.2009.05.003}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1087184509000942}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19460452}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19460452}, day = {19}, doi = {10.1016/j.fgb.2009.05.003}, issn = {1096-0937}, journal = {Fungal genetics and biology : FG \& B}, keywords = {aspergillus, iron}, month = {May}, posted-at = {2009-07-06 16:20:36}, priority = {2}, title = {The interplay between iron and zinc metabolism in Aspergillus fumigatus.}, url = {http://dx.doi.org/10.1016/j.fgb.2009.05.003}, year = {2009} }

TY - JOUR ID - citeulike:4836641 L3 - citeulike-article-id:4836641 N2 - Zinc plays a critical role in a diverse array of biochemical processes. However, excess of zinc is deleterious to cells. Therefore, cells require finely tuned homeostatic mechanisms to balance uptake and storage of zinc. Here we show that iron starvation affects zinc metabolism by downregulating expression of the plasma membrane zinc importer encoding zrfB and upregulating the putative vacuolar zinc transporter-encoding zrcA in Aspergillus fumigatus. Nevertheless, the zinc content of iron-starved mycelia exceeded that of iron replete mycelia, possibly due to unspecific metal uptake induced by iron starvation. In agreement with increased zinc excess and zinc toxicity during iron starvation, deficiency in siderophore-mediated high-affinity iron uptake caused hypersensitivity to zinc. Moreover, an increase of zinc uptake by conditional overexpression of zrfB was more toxic under iron depleted compared to iron replete conditions. This deregulated zinc uptake under iron starvation caused a decrease in heme production and an increase in protoporphyrin IX accumulation. Furthermore, zinc excess impaired production of the extracellular siderophore triacetylfusarinine C but not the intracellular siderophore ferricrocin. Taken together, these data demonstrate a fine tuned coordination of zinc and iron metabolism in A. fumigatus. JF - Fungal genetics and biology : FG & B SN - 1096-0937 TI - The interplay between iron and zinc metabolism in Aspergillus fumigatus. KW - aspergillus KW - iron AU - Yasmin, Sabiha AU - Abt, Beate AU - Schrettl, Markus AU - Moussa, Tarek AU - Werner, Ernst AU - Haas, Hubertus PY - 2009/05/19/ UR - http://dx.doi.org/10.1016/j.fgb.2009.05.003 ER -