Nonhomologous end-joining deficiency allows large chromosomal deletions to be produced by replacement-type recombination in Aspergillus oryae. Export

@article{citeulike:5376069, abstract = {Gene targeting is a technique of introducing a genetic trait at a predetermined site within a genome; it is also used to eliminate undesirable chromosomal regions from the relevant genome. Thus far, replacement-type recombination between 2 homologous regions separated by a large nonhomologous sequence has been hardly achieved probably due to the low frequency of homologous recombination in filamentous fungi. In this study, we report the successful and highly efficient deletion by replacement-type recombination of up to 470-kb regions of chromosome 8 and 200 kb region in chromosome 3, which includes a homologue of aflatoxin gene cluster, by nonhomologous end-joining deficient strains of Aspergillus oryzae. Our study results indicate that the deficiency of nonhomologous end-joining increases the distance of non homologous regions in replacement-type recombination, i.e., the possible deletion range in generation of large chromosomal deletion by one cycle of replacement-type recombination is increased in nonhomologous end-joining deficient strains.}, author = {Takahashi, Tadashi and Jin, Feng Jie J. and Koyama, Yasuji}, citeulike-article-id = {5376069}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.fgb.2009.07.009}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1087184509001364}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19654050}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19654050}, day = {1}, doi = {10.1016/j.fgb.2009.07.009}, issn = {1096-0937}, journal = {Fungal genetics and biology : FG \& B}, keywords = {aspergillus, chromosome}, month = {August}, posted-at = {2009-08-11 15:28:59}, priority = {2}, title = {Nonhomologous end-joining deficiency allows large chromosomal deletions to be produced by replacement-type recombination in Aspergillus oryae.}, url = {http://dx.doi.org/10.1016/j.fgb.2009.07.009}, year = {2009} }

TY - JOUR ID - citeulike:5376069 L3 - citeulike-article-id:5376069 N2 - Gene targeting is a technique of introducing a genetic trait at a predetermined site within a genome; it is also used to eliminate undesirable chromosomal regions from the relevant genome. Thus far, replacement-type recombination between 2 homologous regions separated by a large nonhomologous sequence has been hardly achieved probably due to the low frequency of homologous recombination in filamentous fungi. In this study, we report the successful and highly efficient deletion by replacement-type recombination of up to 470-kb regions of chromosome 8 and 200 kb region in chromosome 3, which includes a homologue of aflatoxin gene cluster, by nonhomologous end-joining deficient strains of Aspergillus oryzae. Our study results indicate that the deficiency of nonhomologous end-joining increases the distance of non homologous regions in replacement-type recombination, i.e., the possible deletion range in generation of large chromosomal deletion by one cycle of replacement-type recombination is increased in nonhomologous end-joining deficient strains. JF - Fungal genetics and biology : FG & B SN - 1096-0937 TI - Nonhomologous end-joining deficiency allows large chromosomal deletions to be produced by replacement-type recombination in Aspergillus oryae. KW - aspergillus KW - chromosome AU - Takahashi, Tadashi AU - Jin, Feng Jie AU - Koyama, Yasuji PY - 2009/08/01/ UR - http://dx.doi.org/10.1016/j.fgb.2009.07.009 ER -