The roles of temperature, pH and mosquito factors as triggers of male and female gametogenesis of Plasmodium berghei in vitro. Export

@article{citeulike:2002955, abstract = {Developmentally arrested malarial gametocytes undergo gamete formation in the mosquito midgut immediately after ingestion of the infected bloodmeal. In the rodent malaria parasite Plasmodium berghei male gametogenesis (exflagellation) can be induced in vitro by a temperature decrease (from 39 degrees C in the vertebrate host to 20 degrees C) and a concomitant pH increase (from 7.3 in mouse blood to 8.0). We report the presence of additional Gametocyte Activating Factor(s) (GAF) present in Anopheles stephensi tissue extracts, which induce both male and female gametogenesis at the otherwise nonpermissive pH of 7.3 in vitro but are unable to overcome the low temperature requirement. All constituent cellular events of microgametogeneis studied here are induced by the same triggers in vitro. A temperature decrease is also required for exflagellation in the mosquito midgut. The possible role of GAF as a second obligatory natural trigger of gametogenesis is discussed.}, address = {Infection and Immunity Section, Department of Biology, Imperial College of Science, Technology and Medicine, London, UK.}, author = {Billker, O. and Shaw, M. K. and Margos, G. and Sinden, R. E.}, citeulike-article-id = {2002955}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9280891}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9280891}, issn = {0031-1820}, journal = {Parasitology}, keywords = {gametocyte}, month = {July}, pages = {1--7}, posted-at = {2007-11-28 10:55:14}, priority = {2}, title = {The roles of temperature, pH and mosquito factors as triggers of male and female gametogenesis of Plasmodium berghei in vitro.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9280891}, volume = {115 ( Pt 1)}, year = {1997} }

TY - JOUR ID - citeulike:2002955 L3 - citeulike-article-id:2002955 N2 - Developmentally arrested malarial gametocytes undergo gamete formation in the mosquito midgut immediately after ingestion of the infected bloodmeal. In the rodent malaria parasite Plasmodium berghei male gametogenesis (exflagellation) can be induced in vitro by a temperature decrease (from 39 degrees C in the vertebrate host to 20 degrees C) and a concomitant pH increase (from 7.3 in mouse blood to 8.0). We report the presence of additional Gametocyte Activating Factor(s) (GAF) present in Anopheles stephensi tissue extracts, which induce both male and female gametogenesis at the otherwise nonpermissive pH of 7.3 in vitro but are unable to overcome the low temperature requirement. All constituent cellular events of microgametogeneis studied here are induced by the same triggers in vitro. A temperature decrease is also required for exflagellation in the mosquito midgut. The possible role of GAF as a second obligatory natural trigger of gametogenesis is discussed. JF - Parasitology SN - 0031-1820 AD - Infection and Immunity Section, Department of Biology, Imperial College of Science, Technology and Medicine, London, UK. EP - 7 TI - The roles of temperature, pH and mosquito factors as triggers of male and female gametogenesis of Plasmodium berghei in vitro. VL - 115 ( Pt 1) SP - 1 KW - gametocyte AU - Billker, O AU - Shaw, MK AU - Margos, G AU - Sinden, RE PY - 1997/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/9280891 ER -