A conserved U-rich RNA region implicated in regulation of translation in Plasmodium female gametocytes. Export

@article{citeulike:2188352, abstract = {Translational repression (TR) plays an important role in post-transcriptional regulation of gene expression and embryonic development in metazoans. TR also regulates the expression of a subset of the cytoplasmic mRNA population during development of fertilized female gametes of the unicellular malaria parasite, Plasmodium spp. which results in the formation of a polar and motile form, the ookinete. We report the conserved and sex-specific regulatory role of either the 3'- or 5'-UTR of a subset of translationally repressed mRNA species as shown by almost complete inhibition of expression of a GFP reporter protein in the female gametocyte. A U-rich, TR-associated element, identified previously in the 3'-UTR of TR-associated transcripts, played an essential role in mediating TR and a similar region could be found in the 5'-UTR shown in this study to be active in TR. The silencing effect of this 5'-UTR was shown to be independent of its position relative to its ORF, as transposition to a location 3' of the ORF did not affect TR. These results demonstrate for the first time in a unicellular organism that the 5' or the 3'-UTR of TR-associated transcripts play an important and conserved role in mediating TR in female gametocytes.}, address = {Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre (LUMC), Leiden, The Netherlands.}, author = {Braks, Joanna A M A. and Mair, Gunnar R R. and Franke-Fayard, Blandine and Janse, Chris J J. and Waters, Andrew P P.}, citeulike-article-id = {2188352}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18158300}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18158300}, day = {23}, issn = {1362-4962}, journal = {Nucleic Acids Res}, keywords = {gametocyte, gene-reg}, month = {December}, posted-at = {2008-01-02 14:06:43}, priority = {2}, title = {A conserved U-rich RNA region implicated in regulation of translation in Plasmodium female gametocytes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18158300}, year = {2007} }

TY - JOUR ID - citeulike:2188352 L3 - citeulike-article-id:2188352 N2 - Translational repression (TR) plays an important role in post-transcriptional regulation of gene expression and embryonic development in metazoans. TR also regulates the expression of a subset of the cytoplasmic mRNA population during development of fertilized female gametes of the unicellular malaria parasite, Plasmodium spp. which results in the formation of a polar and motile form, the ookinete. We report the conserved and sex-specific regulatory role of either the 3'- or 5'-UTR of a subset of translationally repressed mRNA species as shown by almost complete inhibition of expression of a GFP reporter protein in the female gametocyte. A U-rich, TR-associated element, identified previously in the 3'-UTR of TR-associated transcripts, played an essential role in mediating TR and a similar region could be found in the 5'-UTR shown in this study to be active in TR. The silencing effect of this 5'-UTR was shown to be independent of its position relative to its ORF, as transposition to a location 3' of the ORF did not affect TR. These results demonstrate for the first time in a unicellular organism that the 5' or the 3'-UTR of TR-associated transcripts play an important and conserved role in mediating TR in female gametocytes. JF - Nucleic Acids Res SN - 1362-4962 AD - Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre (LUMC), Leiden, The Netherlands. TI - A conserved U-rich RNA region implicated in regulation of translation in Plasmodium female gametocytes. KW - gametocyte KW - gene-reg AU - Braks, Joanna A M AU - Mair, Gunnar R AU - Franke-Fayard, Blandine AU - Janse, Chris J AU - Waters, Andrew P PY - 2007/12/23/ UR - http://view.ncbi.nlm.nih.gov/pubmed/18158300 ER -