The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. Export

@article{citeulike:2610623, abstract = {The cellular and molecular mechanisms that underlie species-specific membrane fusion between male and female gametes remain largely unknown. Here, by use of gene discovery methods in the green alga Chlamydomonas, gene disruption in the rodent malaria parasite Plasmodium berghei, and distinctive features of fertilization in both organisms, we report discovery of a mechanism that accounts for a conserved protein required for gamete fusion. A screen for fusion mutants in Chlamydomonas identified a homolog of HAP2, an Arabidopsis sterility gene. Moreover, HAP2 disruption in Plasmodium blocked fertilization and thereby mosquito transmission of malaria. HAP2 localizes at the fusion site of Chlamydomonas minus gametes, yet Chlamydomonas minus and Plasmodium hap2 male gametes retain the ability, using other, species-limited proteins, to form tight prefusion membrane attachments with their respective gamete partners. Membrane dye experiments show that HAP2 is essential for membrane merger. Thus, in two distantly related eukaryotes, species-limited proteins govern access to a conserved protein essential for membrane fusion.}, address = {Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA;}, author = {Liu, Yanjie and Tewari, Rita and Ning, Jue and Blagborough, Andrew M M. and Garbom, Sara and Pei, Jimin and Grishin, Nick V V. and Steele, Robert E E. and Sinden, Robert E E. and Snell, William J J. and Billker, Oliver}, citeulike-article-id = {2610623}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gad.1656508}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18367645}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18367645}, day = {26}, doi = {10.1101/gad.1656508}, issn = {0890-9369}, journal = {Genes Dev}, keywords = {fusm-hap2}, month = {March}, posted-at = {2008-03-29 12:40:11}, priority = {2}, title = {The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes.}, url = {http://dx.doi.org/10.1101/gad.1656508}, year = {2008} }

TY - JOUR ID - citeulike:2610623 L3 - citeulike-article-id:2610623 N2 - The cellular and molecular mechanisms that underlie species-specific membrane fusion between male and female gametes remain largely unknown. Here, by use of gene discovery methods in the green alga Chlamydomonas, gene disruption in the rodent malaria parasite Plasmodium berghei, and distinctive features of fertilization in both organisms, we report discovery of a mechanism that accounts for a conserved protein required for gamete fusion. A screen for fusion mutants in Chlamydomonas identified a homolog of HAP2, an Arabidopsis sterility gene. Moreover, HAP2 disruption in Plasmodium blocked fertilization and thereby mosquito transmission of malaria. HAP2 localizes at the fusion site of Chlamydomonas minus gametes, yet Chlamydomonas minus and Plasmodium hap2 male gametes retain the ability, using other, species-limited proteins, to form tight prefusion membrane attachments with their respective gamete partners. Membrane dye experiments show that HAP2 is essential for membrane merger. Thus, in two distantly related eukaryotes, species-limited proteins govern access to a conserved protein essential for membrane fusion. JF - Genes Dev SN - 0890-9369 AD - Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA; TI - The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. KW - fusm-hap2 AU - Liu, Yanjie AU - Tewari, Rita AU - Ning, Jue AU - Blagborough, Andrew M AU - Garbom, Sara AU - Pei, Jimin AU - Grishin, Nick V AU - Steele, Robert E AU - Sinden, Robert E AU - Snell, William J AU - Billker, Oliver PY - 2008/03/26/ UR - http://dx.doi.org/10.1101/gad.1656508 ER -