The biosynthesis and post-translational modification of Pbs21 an ookinete-surface protein of Plasmodium berghei.

@article{citeulike:2797803, abstract = {Radiolabelled methionine incorporation into synchronised Plasmodium berghei gametocytes or ookinete cultures, showed that Pbs21 is not synthesised in bloodstage parasites; synthesis was detected within three hours of induction of gametogenesis; synthesis was triggered at gametogenesis, not by fertilisation. We show native Pbs21 to be a hydrophobic membrane protein that was insensitive to cleavage by phosphatidylinositol phospholipase C (PI-PLC), but sensitive to alkaline hydroxylamine, and partially sensitive to glycosylphosphatidylinositol-dependent phospholipase D (GPI-PLD) and HNO2. 3H-myristic and palmitic acid, 3H-glucosamine and mannose incorporation indicated Pbs21 was acylated and glycosylated. Linkage of the acyl group was sensitive to HNO2, which released an acyl-phosphatidylinositol more hydrophobic than that released from P3 of Trypanosoma brucei. All these properties are consistent with the presence of a malaria-specific glycosylphosphatidylinositol (GPI) anchor. In contrast recombinant Pbs21 (rPbs21), expressed in Spodoptera frugiperda cells, was sensitive to both PI-PLC and GPI-PLD, consistent with the protein being modified by a different (S. frugiperda) GPI anchor. Brefeldin A blocked secretion of rPbs21 within a cytoplasmic reticular compartment. Following deletion of the putative GPI anchor addition site (amino acids 189 213), the protein was transported to the cell surface and secreted directly into the aqueous phase of the culture medium. Deletion of amino acids 205-213 disrupted Pbs21 processing, transport through the ER and distribution onto the cell surface. Deletion of amino acids 1-28 prevented transport of Pbs21 into the ER. This suggests that correct processing of the GPI anchor in the ER-Golgi network is essential for the successful secretion of the recombinant protein, which is additionally dependent upon an N-terminal secretory signal sequence.}, address = {Department of Biology, Imperial College of Science, Technology and Medicine, London, UK.}, author = {Blanco, A. R. and Paez, A. and Gerold, P. and Dearsly, A. L. and Margos, G. and Schwarz, R. T. and Barker, G. and Rodriguez, M. C. and Sinden, R. E. }, citeulike-article-id = {2797803}, issn = {0166-6851}, journal = {Molecular and biochemical parasitology}, keywords = {p21}, month = {January}, number = {2}, pages = {163--173}, posted-at = {2008-05-14 12:56:22}, priority = {2}, title = {The biosynthesis and post-translational modification of Pbs21 an ookinete-surface protein of Plasmodium berghei.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10080386}, volume = {98}, year = {1999} }

TY - JOUR ID - citeulike:2797803 L3 - citeulike-article-id:2797803 TI - The biosynthesis and post-translational modification of Pbs21 an ookinete-surface protein of Plasmodium berghei. JF - Molecular and biochemical parasitology VL - 98 IS - 2 SP - 163 EP - 173 AD - Department of Biology, Imperial College of Science, Technology and Medicine, London, UK. SN - 0166-6851 N2 - Radiolabelled methionine incorporation into synchronised Plasmodium berghei gametocytes or ookinete cultures, showed that Pbs21 is not synthesised in bloodstage parasites; synthesis was detected within three hours of induction of gametogenesis; synthesis was triggered at gametogenesis, not by fertilisation. We show native Pbs21 to be a hydrophobic membrane protein that was insensitive to cleavage by phosphatidylinositol phospholipase C (PI-PLC), but sensitive to alkaline hydroxylamine, and partially sensitive to glycosylphosphatidylinositol-dependent phospholipase D (GPI-PLD) and HNO2. 3H-myristic and palmitic acid, 3H-glucosamine and mannose incorporation indicated Pbs21 was acylated and glycosylated. Linkage of the acyl group was sensitive to HNO2, which released an acyl-phosphatidylinositol more hydrophobic than that released from P3 of Trypanosoma brucei. All these properties are consistent with the presence of a malaria-specific glycosylphosphatidylinositol (GPI) anchor. In contrast recombinant Pbs21 (rPbs21), expressed in Spodoptera frugiperda cells, was sensitive to both PI-PLC and GPI-PLD, consistent with the protein being modified by a different (S. frugiperda) GPI anchor. Brefeldin A blocked secretion of rPbs21 within a cytoplasmic reticular compartment. Following deletion of the putative GPI anchor addition site (amino acids 189 213), the protein was transported to the cell surface and secreted directly into the aqueous phase of the culture medium. Deletion of amino acids 205-213 disrupted Pbs21 processing, transport through the ER and distribution onto the cell surface. Deletion of amino acids 1-28 prevented transport of Pbs21 into the ER. This suggests that correct processing of the GPI anchor in the ER-Golgi network is essential for the successful secretion of the recombinant protein, which is additionally dependent upon an N-terminal secretory signal sequence. KW - p21 AU - Blanco, AR AU - Paez, A AU - Gerold, P AU - Dearsly, AL AU - Margos, G AU - Schwarz, RT AU - Barker, G AU - Rodriguez, MC AU - Sinden, RE PY - 1999/01/25/ UR - http://view.ncbi.nlm.nih.gov/pubmed/10080386 ER -