A SPECTROPHOTOMETRIC ASSAY FOR RUBISCO ACTIVITY: APPLICATION TO THE KELP <i>LAMINARIA SACCHARINA</i> AND IMPLICATIONS FOR RADIOMETRIC ASSAYS¹ Export

@article{citeulike:3578305, abstract = {We optimized a spectrophotometric method for measuring ribulose-l,5-bisphosphate carboxylase oxygenase (Rubisco) activity in crude extracts of the kelp Laminaria saccharina Lamour. Activity exhibited a decline during the assays, such that rates determined over a 10-min period were only 70\% of activity during the first minute. Activity was significantly enhanced by addition of HCO32212 to the extraction buffer and was stable for at least 4 h. Highest activities were determined using small extract loads (120132.5 mg fresh weight·mL22121 of assay mixture); extract loads of 10 mg·mL22121caused a 25\% decrease in activity. Rubisco activities determined for individual kelp plants using the spectrophotometric method were significantly correlated with total protein and gross photo synthetic capacity (Pmax) and were 1.520132 times higher than Pmax. Low Rubisco activities relative to photosynthetic rates reported in many previous studies of macroalgae and microalgae may have resulted at least partly from underestimation of enzyme activity by radiometric assays using relatively large volumes of un-activated crude extract and assay periods longer than 1 min.}, address = {Marine Sciences Research Center, State University of New York, Stony Brook, New York 11794-5000}, author = {Gerard, Valrie A. and Driscoll, Timothy}, citeulike-article-id = {3578305}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1529-8817.1996.tb02190.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/119951988/ABSTRACT}, doi = {10.1111/j.1529-8817.1996.tb02190.x}, journal = {Journal of Phycology}, keywords = {algae, biology, kelp, laminaria, marine, photosynthesis, protein, rubisco, spectrophotometry}, number = {5}, pages = {880--884}, posted-at = {2008-11-19 14:58:43}, priority = {0}, title = {A SPECTROPHOTOMETRIC ASSAY FOR RUBISCO ACTIVITY: APPLICATION TO THE KELP <i>LAMINARIA SACCHARINA</i> AND IMPLICATIONS FOR RADIOMETRIC ASSAYS¹}, url = {http://dx.doi.org/10.1111/j.1529-8817.1996.tb02190.x}, volume = {32}, year = {1996} }

TY - JOUR ID - citeulike:3578305 L3 - citeulike-article-id:3578305 N2 - We optimized a spectrophotometric method for measuring ribulose-l,5-bisphosphate carboxylase oxygenase (Rubisco) activity in crude extracts of the kelp Laminaria saccharina Lamour. Activity exhibited a decline during the assays, such that rates determined over a 10-min period were only 70% of activity during the first minute. Activity was significantly enhanced by addition of HCO32212 to the extraction buffer and was stable for at least 4 h. Highest activities were determined using small extract loads (120132.5 mg fresh weight·mL22121 of assay mixture); extract loads of 10 mg·mL22121caused a 25% decrease in activity. Rubisco activities determined for individual kelp plants using the spectrophotometric method were significantly correlated with total protein and gross photo synthetic capacity (Pmax) and were 1.520132 times higher than Pmax. Low Rubisco activities relative to photosynthetic rates reported in many previous studies of macroalgae and microalgae may have resulted at least partly from underestimation of enzyme activity by radiometric assays using relatively large volumes of un-activated crude extract and assay periods longer than 1 min. IS - 5 JF - Journal of Phycology AD - Marine Sciences Research Center, State University of New York, Stony Brook, New York 11794-5000 EP - 884 TI - A SPECTROPHOTOMETRIC ASSAY FOR RUBISCO ACTIVITY: APPLICATION TO THE KELP <i>LAMINARIA SACCHARINA</i> AND IMPLICATIONS FOR RADIOMETRIC ASSAYS¹ VL - 32 SP - 880 KW - algae KW - biology KW - kelp KW - laminaria KW - marine KW - photosynthesis KW - protein KW - rubisco KW - spectrophotometry AU - Gerard, Valrie AU - Driscoll, Timothy PY - 1996/// UR - http://dx.doi.org/10.1111/j.1529-8817.1996.tb02190.x ER -