A new enzymatic approach for estimating total and direct bilirubin. Export

@article{citeulike:1219199, abstract = {We designed an enzymatic assay for total (TBil) and direct bilirubin (DBil), the principle of which involves measuring the decrease in absorbance at 450 nm produced by bilirubin oxidase from Myrothecium verrucaria. Since TBil and DBil are oxidized at pH 7.2 and 3.7, respectively, the degree of bilirubin oxidation is measurable in each case. An analysis of bilirubin by high-performance liquid chromatography, before and after the enzymatic reaction with bilirubin oxidase, verified the specificity of the enzyme. The results obtained using this method varied linearly with TBil and DBil concentrations up to at least 250 mg/L and 150 mg/L, respectively. Reducing substances, commonly used anticoagulants and hemoglobin showed no apparent interference. The degree of day-to-day precision (CV) for TBil and DBil ranged from 1.2\% (206.2 mg/L) to 10.6\% (3.5 mg/L) and from 1.8\% (84.3 mg/L) to 12.4\% (2.1 mg/L), respectively. Values measured using this new method correlated well with those obtained by Malloy-Evelyn's method and the slide method employing the Kodak Ektachem analyzer.}, address = {Department of Laboratory Medicine, Faculty of Medicine, Kagoshima University, Japan.}, author = {Otsuji, S. and Mizuno, K. and Ito, S. and Kawahara, S. and Kai, M.}, citeulike-article-id = {1219199}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/3345597}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=3345597}, issn = {0009-9120}, journal = {Clin Biochem}, keywords = {bilirubin, oxidase}, month = {January}, number = {1}, pages = {33--38}, posted-at = {2007-04-10 16:26:17}, priority = {2}, title = {A new enzymatic approach for estimating total and direct bilirubin.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3345597}, volume = {21}, year = {1988} }

TY - JOUR ID - citeulike:1219199 L3 - citeulike-article-id:1219199 N2 - We designed an enzymatic assay for total (TBil) and direct bilirubin (DBil), the principle of which involves measuring the decrease in absorbance at 450 nm produced by bilirubin oxidase from Myrothecium verrucaria. Since TBil and DBil are oxidized at pH 7.2 and 3.7, respectively, the degree of bilirubin oxidation is measurable in each case. An analysis of bilirubin by high-performance liquid chromatography, before and after the enzymatic reaction with bilirubin oxidase, verified the specificity of the enzyme. The results obtained using this method varied linearly with TBil and DBil concentrations up to at least 250 mg/L and 150 mg/L, respectively. Reducing substances, commonly used anticoagulants and hemoglobin showed no apparent interference. The degree of day-to-day precision (CV) for TBil and DBil ranged from 1.2% (206.2 mg/L) to 10.6% (3.5 mg/L) and from 1.8% (84.3 mg/L) to 12.4% (2.1 mg/L), respectively. Values measured using this new method correlated well with those obtained by Malloy-Evelyn's method and the slide method employing the Kodak Ektachem analyzer. IS - 1 JF - Clin Biochem SN - 0009-9120 AD - Department of Laboratory Medicine, Faculty of Medicine, Kagoshima University, Japan. EP - 38 TI - A new enzymatic approach for estimating total and direct bilirubin. VL - 21 SP - 33 KW - bilirubin KW - oxidase AU - Otsuji, S AU - Mizuno, K AU - Ito, S AU - Kawahara, S AU - Kai, M PY - 1988/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/3345597 ER -