Basic and clinical evaluation of a newly-developed enzymatic bilirubin assay. Export

@article{citeulike:1219200, abstract = {An enzymatic bilirubin assay using bilirubin oxidase was evaluated. Its repeatability and linearity were excellent, and excellent correlations were observed between the enzyme and the diazo methods in total and conjugated bilirubin. The coefficient of correlation between the conjugated bilirubin fraction obtained with the diazo method and that of HPLC was 0.424, while this coefficient was 0.580 for the diazo method. A multi-laboratory study using 3 serum specimens revealed lower coefficients of variation for the enzymatic method than for the diazo method. Bilirubinuria specimen showed good accord between total bilirubin and conjugate bilirubin levels for the enzymatic method, not for the diazo method. When 12 newborn baby specimens and 34 pediatric specimens were studied, all exhibited increased concentrations of conjugated bilirubin with the diazo method. In an adult case with prolonged jaundice, the diazo method usually yielded higher conjugated bilirubin values than the enzymatic method. The enzymatic method was then concluded to be superior to the diazo method for conjugated bilirubin determination.}, address = {Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.}, author = {Kirihigashi, K. and Tatsumi, N. and Hino, M. and Yamane, T. and Ohta, K.}, citeulike-article-id = {1219200}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10983467}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10983467}, issn = {0030-6096}, journal = {Osaka City Med J}, keywords = {bilirubin, oxidase}, month = {June}, number = {1}, pages = {55--70}, posted-at = {2007-04-10 16:26:43}, priority = {2}, title = {Basic and clinical evaluation of a newly-developed enzymatic bilirubin assay.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10983467}, volume = {46}, year = {2000} }

TY - JOUR ID - citeulike:1219200 L3 - citeulike-article-id:1219200 N2 - An enzymatic bilirubin assay using bilirubin oxidase was evaluated. Its repeatability and linearity were excellent, and excellent correlations were observed between the enzyme and the diazo methods in total and conjugated bilirubin. The coefficient of correlation between the conjugated bilirubin fraction obtained with the diazo method and that of HPLC was 0.424, while this coefficient was 0.580 for the diazo method. A multi-laboratory study using 3 serum specimens revealed lower coefficients of variation for the enzymatic method than for the diazo method. Bilirubinuria specimen showed good accord between total bilirubin and conjugate bilirubin levels for the enzymatic method, not for the diazo method. When 12 newborn baby specimens and 34 pediatric specimens were studied, all exhibited increased concentrations of conjugated bilirubin with the diazo method. In an adult case with prolonged jaundice, the diazo method usually yielded higher conjugated bilirubin values than the enzymatic method. The enzymatic method was then concluded to be superior to the diazo method for conjugated bilirubin determination. IS - 1 JF - Osaka City Med J SN - 0030-6096 AD - Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan. EP - 70 TI - Basic and clinical evaluation of a newly-developed enzymatic bilirubin assay. VL - 46 SP - 55 KW - bilirubin KW - oxidase AU - Kirihigashi, K AU - Tatsumi, N AU - Hino, M AU - Yamane, T AU - Ohta, K PY - 2000/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/10983467 ER -