Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine Export

@article{citeulike:2290664, abstract = {A novel electrogenerated chemiluminescence aptamer-based (ECL-AB) biosensor for the determination of a small molecule drug is designed employing cocaine-binding aptamer as molecular recognition element for cocaine as a model analyte and ruthenium complex served as an ECL label. A 5'-terminal cocaine-binding aptamer with the ECL label at 3'-terminal of the aptamer was utilized as an ECL probe. The ECL-AB biosensors were fabricated by immobilizing the ECL probe onto a gold electrode surface via thiol-Au interactions. An enhanced ECL signal is generated upon recognition of the target cocaine, attributed to a change in the conformation of the ECL probe from random coil-like configuration on the probe-modified film to three-way junction structure, in close proximity to the sensor interface. The integrated ECL intensity versus the concentration of cocaine was linear in the range from 5.0 x 10-9 to 3.0 x 10-7 M. The detection limit was 1.0 x 10-9 M. This work demonstrates that the combination of a highly binding aptamer to analyte with a highly sensitive ECL technique to design ECL-AB biosensor is a great promising approach for the determination of small molecule drugs.}, author = {Li, Yan and Qi, Honglan and Peng, Yage and Yang, Jia and Zhang, Chengxiao}, citeulike-article-id = {2290664}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.elecom.2007.07.038}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6VP5-4PCGS4X-1/2/89a0762d9b87f6418c7113eb3bd876ca}, doi = {10.1016/j.elecom.2007.07.038}, journal = {Electrochemistry Communications}, keywords = {chemiluminescence, electrogenerated}, month = {October}, number = {10}, pages = {2571--2575}, posted-at = {2008-01-25 16:53:38}, priority = {2}, title = {Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine}, url = {http://dx.doi.org/10.1016/j.elecom.2007.07.038}, volume = {9}, year = {2007} }

TY - JOUR ID - citeulike:2290664 L3 - citeulike-article-id:2290664 N2 - A novel electrogenerated chemiluminescence aptamer-based (ECL-AB) biosensor for the determination of a small molecule drug is designed employing cocaine-binding aptamer as molecular recognition element for cocaine as a model analyte and ruthenium complex served as an ECL label. A 5'-terminal cocaine-binding aptamer with the ECL label at 3'-terminal of the aptamer was utilized as an ECL probe. The ECL-AB biosensors were fabricated by immobilizing the ECL probe onto a gold electrode surface via thiol-Au interactions. An enhanced ECL signal is generated upon recognition of the target cocaine, attributed to a change in the conformation of the ECL probe from random coil-like configuration on the probe-modified film to three-way junction structure, in close proximity to the sensor interface. The integrated ECL intensity versus the concentration of cocaine was linear in the range from 5.0 x 10-9 to 3.0 x 10-7 M. The detection limit was 1.0 x 10-9 M. This work demonstrates that the combination of a highly binding aptamer to analyte with a highly sensitive ECL technique to design ECL-AB biosensor is a great promising approach for the determination of small molecule drugs. IS - 10 JF - Electrochemistry Communications EP - 2575 TI - Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine VL - 9 SP - 2571 KW - chemiluminescence KW - electrogenerated AU - Li, Yan AU - Qi, Honglan AU - Peng, Yage AU - Yang, Jia AU - Zhang, Chengxiao PY - 2007/10// UR - http://dx.doi.org/10.1016/j.elecom.2007.07.038 ER -