Homogeneous enzyme immunoassay for proteins employing [beta]-galactosidase Export

@article{citeulike:2374331, abstract = {A homogeneous enzyme immunoassay method for proteins is described. The assay components include a protein antigen--[beta]-galactosidase conjugate, antiprotein antibody, and a synthetic macromolecular substrate. When antibody binds to the conjugate, enzyme activity is inhibited up to 95\% because of steric exclusion of the substrate. Free-protein antigen competes for antibody, thus preventing inhibition. The extent of inhibition is related to the concentration of protein analyte. The assay is simple to perform, highly sensitive, and is generally useful for proteins with wide structural variations.}, author = {Gibbons, Ian and Skold, Carl and Rowley, Gerald L. and Ullman, Edwin F.}, citeulike-article-id = {2374331}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0003-2697(80)90334-6}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/0003269780903346}, citeulike-linkout-2 = {http://www.sciencedirect.com/science/article/B6W9V-4DYTNJ8-11C/2/787aeb2b62d167dab65ecce58334fa2b}, doi = {10.1016/0003-2697(80)90334-6}, journal = {Analytical Biochemistry}, keywords = {beta-gal, emit, homogeneous, immunoassay, protein}, month = {February}, number = {1}, pages = {167--170}, posted-at = {2008-12-01 14:17:40}, priority = {2}, title = {Homogeneous enzyme immunoassay for proteins employing [beta]-galactosidase}, url = {http://dx.doi.org/10.1016/0003-2697(80)90334-6}, volume = {102}, year = {1980} }

TY - JOUR ID - citeulike:2374331 L3 - citeulike-article-id:2374331 N2 - A homogeneous enzyme immunoassay method for proteins is described. The assay components include a protein antigen--[beta]-galactosidase conjugate, antiprotein antibody, and a synthetic macromolecular substrate. When antibody binds to the conjugate, enzyme activity is inhibited up to 95% because of steric exclusion of the substrate. Free-protein antigen competes for antibody, thus preventing inhibition. The extent of inhibition is related to the concentration of protein analyte. The assay is simple to perform, highly sensitive, and is generally useful for proteins with wide structural variations. IS - 1 JF - Analytical Biochemistry EP - 170 TI - Homogeneous enzyme immunoassay for proteins employing [beta]-galactosidase VL - 102 SP - 167 KW - beta-gal KW - emit KW - homogeneous KW - immunoassay KW - protein AU - Gibbons, Ian AU - Skold, Carl AU - Rowley, Gerald AU - Ullman, Edwin PY - 1980/02// UR - http://dx.doi.org/10.1016/0003-2697(80)90334-6 ER -